Phosphorylation of epidermal keratins.
Phosphorylation of epidermal keratins.
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表皮角蛋白的磷酸化。
DOI：
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发表时间：
1980
影响因子：
6.5
通讯作者：
I. Freedberg
中科院分区：
文献类型：
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作者：
M. Gilmartin;V. Culbertson;I. Freedberg
当人类和大鼠表皮暴露于32 P-正磷酸时，标记的磷酸盐被掺入到几种蛋白质中。磷酸化的模式是相同的，无论是在体内或体外的同位素交付。主要的磷酸化蛋白不溶于Tris-HCl缓冲液，但可溶于SDS-β-巯基乙醇。它们在SDS-聚丙烯酰胺凝胶中迁移，表观分子量在45，000和65，000之间。当通过二维凝胶电泳分析时，标记的磷蛋白与从人愈伤组织分离的角蛋白共迁移。丝氨酸是这些蛋白质中的磷酸受体。这些SDS-β-巯基乙醇可溶性表皮蛋白的磷酸化模式在基底细胞癌、寻常性鱼鳞病、Kyrle病或内瑟顿综合征中没有改变。牛皮癣的症状有哪些？其他人已经证明，63，000分子量的蛋白质不存在于活动性银屑病病变中。我们发现，63，000分子量的磷蛋白存在于未受累的皮肤中，但在银屑病斑块中不存在。
When human and rat epidermis are exposed to 32P-orthophosphoric acid, labeled phosphate is incorporated into several proteins. The pattern of phosphorylation is identical whether the isotope is delivered in vivo or in vitro. The predominant phosphorylated proteins are insoluble in Tris-HCl buffer but soluble in SDS-beta-mercaptoethanol. They migrate in SDS-polyacrylamide gels with apparent molecular weights between 45,000 and 65,000. When analyzed by two-dimensional gel electrophoresis, the labeled phosphoproteins co-migrate with keratins isolated from human callus. Serine is the phosphate acceptor in these proteins. The pattern of phosphorylation of these SDS-beta-mercaptoethanol soluble epidermal proteins is not changed in basal cell carcinoma, icthyosis vulgaris, Kyrle's disease or Netherton's syndrome. The pattern is altered in psoriasis. Others have demonstrated that a 63,000 molecular weight protein is absent from active psoriatic lesions. We have found that a 63,000 molecular weight phosphoprotein is present in uninvolved skin but absent in the psoriatic plaques.