Isolation and Expression analysis of OsPME1, encoding for a putative Pectin Methyl Esterase from Oryza sativa (subsp indica)
Isolation and Expression analysis of OsPME1, encoding for a putative Pectin Methyl Esterase from Oryza sativa (subsp indica)
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DOI：
10.1007/s12298-009-0014-x
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发表时间：
2009-04-01
影响因子：
3.5
通讯作者：
Gupta, Aditya Kumar
中科院分区：
文献类型：
--
作者：
Kanneganti, Vydehi;Gupta, Aditya Kumar
果胶甲基酯酶（PMEs）通过影响植物细胞壁的力学性质在植物发育过程中起着重要作用。近年来的研究表明，花粉母细胞在花粉管发育过程中起着重要作用。本研究从水稻幼穗cDNA文库中分离到一个1.3kb的cDNA克隆。该基因含有一个1038 bp的开放阅读框（ORF），编码一个345个氨基酸的果胶甲酯酶，并含有一个20个氨基酸的信号肽，命名为OsPME 1（Oryza sativa Pectin Methyl Esterase 1）。它包含的结构安排GXYXE和GXXDFIF，发现在所有PMEs的活性基团。OsPME 1基因产物与其它豆科、豆科、苋科和Funariaceae植物的PMEs同源性在52%~ 33%之间。Southern杂交分析表明，PME 1在水稻基因组中以单拷贝形式存在。表达模式分析表明，OsPME 1只在花粉粒中表达，在其发展的后期阶段，也受到各种非生物胁迫处理和植物激素的调节。水稻花粉特异性PME的功能特性将使我们能够了解它在花粉发育中的作用。
Pectin Methyl Esterases (PMEs) play an essential role during plant development by affecting the mechanical properties of the plant cell walls. Recent studies indicated that PMEs play important role in pollen tube development. In this study, we isolated a 1.3 kb cDNA clone from rice panicle cDNA library. It contained a 1038 bp of open reading frame (ORF) encoding for a putative pectin methyl esterase of 345 aminoacids with a 20 aminoacid signal peptide and was hence designated as OsPME1 (Oryza s ativa Pectin Methyl Esterase 1). It contained the structural arrangement GXYXE and GXXDFIF, found in the active groups of all PMEs. OsPME1 gene product shared varying identities, ranging from 52 % to 33 % with PMEs from other plant species belonging to Brassicaceae, Fabaceae, Amaranthaceae and Funariaceae. Southern blot analysis indicated that PME1 exists as a single copy in the rice genome. Expression pattern analysis revealed that OsPME1 is expressed only in pollen grains, during the later stages of their development and was also regulated by various abiotic stress treatments and phytohormones. Functional characterization of this pollen specific PME from rice would enable us to understand its role in pollen development.