Dual primer emulsion PCR for next- generation DNA sequencing.
Dual primer emulsion PCR for next- generation DNA sequencing.
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DOI：
10.2144/000113423
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发表时间：
2010-05
期刊：
影响因子：
2.7
通讯作者：
Edwards JS
中科院分区：
文献类型：
--
作者：
Xu MY;Aragon AD;Mascarenas MR;Torrez-Martinez N;Edwards JS
We have developed a highly sensitive single-molecule clonal amplification method called dual primer emulsion PCR (DPePCR) for next-generation DNA sequencing. The approach is similar in concept to standard emulsion PCR; however, in DPePCR both primers are attached to the beads, therefore following PCR amplification, both strands of the PCR products are attached to the beads. The ends of each strand can be freed for analysis by restriction digestion of the bridged PCR fragments, which allows efficient paired-end sequencing of fragment libraries.
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影响因子：
56.9
作者：
Shendure, J;Porreca, GJ;Church, GM
通讯作者：
Church, GM
DOI：
10.1073/pnas.1133470100
发表时间：
2003-07-22
影响因子：
11.1
作者：
Dressman, D;Yan, H;Vogelstein, B
通讯作者：
Vogelstein, B
影响因子：
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作者：
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通讯作者：
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作者：
Tawfik, DS;Griffiths, AD
通讯作者：
Griffiths, AD