Measurement of adsorption isotherms by liquid chromatography.
Measurement of adsorption isotherms by liquid chromatography.
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DOI：
10.1016/s0021-9673(00)96140-5
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
J. Jacobson;J. Frenz;Csaba. Horvath
中科院分区：
文献类型：
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作者：
J. Jacobson;J. Frenz;Csaba. Horvath
采用前沿色谱法和平台洗脱法研究了硅基烃类吸附剂在高效液相色谱中的吸附行为，建立了数据采集、分析和关联的方法。测定了所选化合物的等温线，包括几种生物相关性化合物。比较了各种动态等温线测量方法的优缺点，并通过实验结果加以说明。结果表明，前沿分析技术比其他方法更准确、更方便。建立了一个小型化液相色谱系统，通过前沿展开法测量毫克量物质的等温线。该装置的性能与具有在高效液相色谱中常见的尺寸并且需要更大量的物质用于等温线测量的设备的性能相比是有利的。因此，小型化系统非常适合于感兴趣的物质短缺时的应用，如许多生物物质的情况。考察了操作参数对吸附行为的影响，以优化工艺流程，并进一步探索该工艺的基本原理。
Adsorption behavior on silica-bound hydrocarbonaceous sorbents used in high-performance liquid chromatography was investigated by frontal chromatography and elution on a plateau, and procedures were developed for data collection, analysis and correlation. Isotherms of selected compounds were measured, including several of biological relevance. The relative merits of various dynamic methods of isotherm measurement were compared and illustrated by experimental findings. The frontal analysis technique was determined to be more accurate and convenient than other methods. A miniaturized liquid chromatographic system was constructed to measure isotherms with milligram quantities of material by frontal development. The performance of this apparatus compared favorably with that of equipment which has dimensions usual in high-performance liquid chromatography and requires orders of magnitude greater amounts of a substance for isotherm measurement. Thus the miniaturized system is eminently suited for applications when the substance of interest is in short supply, as is the case with many biological substances. The effects of operating parameters on adsorption behavior were investigated, both to optimize the procedure, and to further explore the fundamental of the process.