An integrated green biorefinery approach towards simultaneous recovery of pectin and polyphenols coupled with bioethanol production from waste pomegranate peels
An integrated green biorefinery approach towards simultaneous recovery of pectin and polyphenols coupled with bioethanol production from waste pomegranate peels
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DOI：
10.1016/j.biortech.2018.06.072
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发表时间：
2018-10-01
影响因子：
11.4
通讯作者：
Arora, Amit
中科院分区：
文献类型：
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作者：
Talekar, Sachin;Patti, Antonio F.;Arora, Amit
以废石榴皮（WPP）为原料，采用水热法建立了一个综合生物精炼厂，在生产生物乙醇的同时，无酸无有机溶剂地回收果胶和酚类物质。采用Box-Behnken试验设计优化了水热处理工艺，在115 ℃、液固比为10. 0、水热处理40 min时，果胶和酚类物质的回收率分别为18.8-20.9%和10.6-11.8%。通过红外光谱、核磁共振氢谱和热重分析对WPP果胶进行了表征，结果表明WPP果胶与市售果胶相似。根据WPP品种类型，果胶的酯化度、半乳糖醛酸含量和分子量分别在68- 74%、71- 72%和131，137 - 141，538 Da的范围内。回收的酚类物质含有57-60%的安石榴苷。HT可提高WPP的酶消化率，每kg干物质产生177 g葡萄糖，发酵可获得80 g乙醇，理论产率为88%。
An integrated biorefinery, incorporating hydrothermal processing of waste pomegranate peels (WPP), was proposed for the acid and organic solvent-free simultaneous recovery of pectin and phenolics with bioethanol production. The hydrothermal treatment (HT) was optimized using Box-Behnken design and the maximum recovery of pectin (18.8-20.9%) and phenolics (10.6-11.8%) were obtained by hydrothermal treatment at 115 degrees C for 40 min with a liquid-solid ratio of 10. The WPP pectin was characterized by IR, H-1 NMR, and TGA which showed close similarity to commercial pectin. Depending on WPP cultivar type the degree of esterification, galacturonic acid content and molecular weight of pectin were in the range of 68-74%, 71-72%, and 131,137-141,538 Da, respectively. The recovered phenolics contained 57-60% punicalagin. Enzyme digestibility of WPP improved using HT with 177 g glucose produced per kg dry mass which was fermented to obtain 80 g ethanol with 88% of theoretical yield.