A Simple Method for Constructing Artificial Promoters Activated in Response to Ultrasound Stimulation
A Simple Method for Constructing Artificial Promoters Activated in Response to Ultrasound Stimulation
复制标题
构建响应超声刺激而激活的人工启动子的简单方法
DOI：
10.1007/978-1-4939-7223-4_14
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Kondo Takashi
中科院分区：
文献类型：
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作者：
Ogawa Ryohei;Kagiya Go;Watanabe Akihiko;Morii Akihiro;Cui Zheng-Guo;Kondo Takashi
已经指出，超声可以用作生物过程的控制器，包括基因表达，因为它的能量可以无创地到达身体深处。基因表达可以通过超声进行时间和空间控制，从而以及时的方式为靶组织提供必要的生物活性蛋白。虽然基因表达调控涉及许多过程，但转录是其中最重要的过程之一，而启动子在其中起着至关重要的作用，已知有几种启动子在超声辐照下被激活。然而，在我们看来，人工启动子更适合于临床使用。我们在此描述了通过随机组合顺式元件来构建对超声辐射有响应的启动子的简单方法 （转录因子结合基序），从而提高其对超声辐射的反应性。
It has been pointed out that ultrasound could be used as a controller for bioprocesses including gene expression since its energy can noninvasively reach deep in the body. Gene expression may be timely and spatially controlled by ultrasound, thus providing necessary bioactive proteins for the targeted tissue in a timely fashion. Although there are many processes involved in gene expression control, one of the most important processes is transcription, and the promoter plays an essential role in it. There are several promoters known to be activated in response to ultrasound irradiation. However, in our opinion, an artificial promoter is more suitable for clinical use. We herein describe simple methods to construct promoters that are responsive to ultrasound irradiation by randomly combiningcis-elements (transcription factor binding motifs) and thereby improve its reactivity to ultrasound irradiation.