Treatment with antibiotics that interfere with peptidoglycan biosynthesis inhibits chloroplast division in the desmid Closterium.
Treatment with antibiotics that interfere with peptidoglycan biosynthesis inhibits chloroplast division in the desmid Closterium.
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DOI：
10.1371/journal.pone.0040734
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Takano H
中科院分区：
文献类型：
--
作者：
Matsumoto H;Takechi K;Sato H;Takio S;Takano H
绿藻是一种与陆地植物密切相关的绿藻群。我们研究了干扰肽聚糖生物合成的抗生素对过寒性Closterium peracerosum-strigosum-littorale复合体叶绿体分裂的影响。我们用秋水仙碱检测刚分裂后的细胞，秋水仙碱能抑制Closterium细胞分裂后的伸长。正常的Closterium细胞在分裂前后有两个叶绿体，而氨苄西林、d -环丝氨酸或磷霉素处理的细胞在分裂后只有一个叶绿体，说明细胞分裂时没有叶绿体分裂。抗菌药物杆菌肽和万古霉素无明显作用。电镜观察显示，氨苄西林处理后的Closterium细胞叶绿体呈不规则形状。由于抗生素治疗导致长细胞出现不规则叶绿体和细胞死亡，我们在培养中计算细胞类型。结果表明：先出现一个叶绿体细胞，然后产生一个巨大的叶绿体，抑制细胞分裂，导致细胞伸长，然后死亡。
Charophytes is a green algal group closely related to land plants. We investigated the effects of antibiotics that interfere with peptidoglycan biosynthesis on chloroplast division in the desmid Closterium peracerosum–strigosum–littorale complex. To detect cells just after division, we used colchicine, which inhibits Closterium cell elongation after division. Although normal Closterium cells had two chloroplasts before and after cell division, cells treated with ampicillin, D-cycloserine, or fosfomycin had only one chloroplast after cell division, suggesting that the cells divided without chloroplast division. The antibiotics bacitracin and vancomycin showed no obvious effect. Electron microscopic observation showed that irregular-shaped chloroplasts existed in ampicillin-treated Closterium cells. Because antibiotic treatments resulted in the appearance of long cells with irregular chloroplasts and cell death, we counted cell types in the culture. The results suggested that cells with one chloroplast appeared first and then a huge chloroplast was generated that inhibited cell division, causing elongation followed by cell death.
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影响因子：
64.8
作者：
Matsuzaki, M;Misumi, O;Kuroiwa, T
通讯作者：
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影响因子：
2.9
作者：
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通讯作者：
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DOI：
10.1073/pnas.0510693103
发表时间：
2006-04-25
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
3.4
作者：
Hedges, SB;Blair, JE;Shoe, JL
通讯作者：
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影响因子：
3.4
作者：
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