Purification and identification of neuromedin U as an endogenous ligand for an orphan receptor GPR66 (FM3)
Purification and identification of neuromedin U as an endogenous ligand for an orphan receptor GPR66 (FM3)
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DOI：
10.1006/bbrc.2000.3502
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发表时间：
2000-09-24
影响因子：
3.1
通讯作者：
Kangawa, K
中科院分区：
文献类型：
--
作者：
Kojima, M;Haruno, R;Kangawa, K
GPR66是一种孤儿g蛋白偶联受体（GPCR），其结构与胃饥饿素（ghrelin）和胃动素（motilin）受体相似，我们试图在大鼠组织中纯化GPR66的天然配体，并鉴定了一个23个氨基酸的肽作为内源性配体，序列分析表明该肽为神经蛋白U (NMU)，这是我们小组首次从猪脊髓中纯化的平滑肌收缩肽。NMU与表达GPR66的细胞结合，具有高特异性，可诱导细胞内钙动员。将NMU注入大鼠脑室（ICV）后，能有效抑制食物摄入。相比之下，注射nmu抗体的ICV增加了进食量。这些结果表明NMU是一种有效的内源性厌食肽。(C) 2000年学术出版社。
GPR66 is an orphan G-protein-coupled receptor (GPCR) whose structure is similar to the ghrelin and motilin receptors, We have tried to purify a natural ligand for GPR66 in rat tissues and identified a 23-amino-acid peptide as the endogenous ligand, Sequence analysis revealed the peptide as neuromedin U (NMU), a smooth-muscle-contracting peptide that was first purified from porcine spinal cord by our group. NMU binds to GPR66 expressing cells with: high specificity to induce intracellular calcium mobilization, When NMU was injected intracerebroventricularly (ICV) into rats, it potently suppressed food intake. In contrast, ICV injection of NMU-antibody increased food intake. These results suggest that NMU is a potent endogenous anorexic peptide. (C) 2000 Academic Press.