Matrix metalloproteinase 12 expression is associated with tumor FOXP3+ regulatory T cell infiltration and poor prognosis in hepatocellular carcinoma
Matrix metalloproteinase 12 expression is associated with tumor FOXP3+ regulatory T cell infiltration and poor prognosis in hepatocellular carcinoma
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基质金属蛋白酶 12 表达与肝细胞癌中肿瘤 FOXP3( ) 调节性 T 细胞浸润和不良预后相关
DOI：
10.3892/ol.2018.8642
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发表时间：
2018-07-01
期刊：
影响因子：
2.9
通讯作者：
Shi, Ming
中科院分区：
文献类型：
--
作者：
He, Min-Ke;Le, Yong;Shi, Ming
肝细胞癌（HCC）是世界范围内最致命的癌症类型之一，由于其高复发率和转移率。HCC进展中涉及的分子过程需要进一步研究，以确定用于诊断和治疗的生物标志物。本研究旨在探讨基质金属肽酶12（MMP 12）在人肝癌中的表达及其与预后的关系。采用逆转录-定量聚合酶链反应分析42对手术治疗后HCC患者的肿瘤和非肿瘤肝组织中MMP 12 mRNA的表达。采用免疫组化法检测158例石蜡包埋的肝癌组织中MMP 12和FOXP 3的表达。采用Kaplan-Meier分析和考克斯比例风险模型确定MMP 12表达的预后价值。MMP 12 mRNA在肝肿瘤组织中的表达水平显著高于相应的非肿瘤肝组织。MMP 12表达和FOXP 3.调节性T细胞（Treg）浸润与淋巴结转移呈正相关（r=0.302; P
Hepatocellular carcinoma (HCC) is among the most fatal types of cancer worldwide due to its high rates of recurrence and metastasis. The molecular processes involved in HCC progression require further investigation to identify biomarkers for use in diagnosis and treatment. In the present study, the significance and prognostic value of matrix metallopeptidase 12 (MMP12) expression in human HCC was investigated. MMP12 mRNA expression was investigated using reverse transcription-quantitative polymerase chain reaction analysis of 42 pairs of tumor and non-tumor liver tissues obtained from patients with HCC following surgical treatment. Immunohistochemical staining was used to detect MMP12 and forkhead box P3 (FOXP3) expression in 158 paraffin-embedded HCC tissues. The prognostic value of MMP12 expression was determined using Kaplan-Meier analysis and the Cox proportional hazards model. MMP12 mRNA levels were significantly higher in liver tumor tissues compared with matched non-tumor liver tissues. MMP12 expression and FOXP3 . regulatory T cell (Treg) infiltration was positively correlated (r=0.302; P