Characterization of integrons and antimicrobial resistance genes in clinical isolates of Gram-negative bacteria from Palestinian hospitals
Characterization of integrons and antimicrobial resistance genes in clinical isolates of Gram-negative bacteria from Palestinian hospitals
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DOI：
10.1111/j.1348-0421.2009.00168.x
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发表时间：
2009-11-01
影响因子：
2.6
通讯作者：
Shimamoto, Tadashi
中科院分区：
文献类型：
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作者：
Hussein, Amjad I. A.;Ahmed, Ashraf M.;Shimamoto, Tadashi
2006 年，从巴勒斯坦医院收集了 60 株革兰氏阴性细菌分离株。其中 32 株（53.3%）分离株表现出多重耐药表型。 PCR 和 DNA 测序用于表征整合子和抗菌素耐药基因。 PCR 筛查显示，19 株（31.7%）和 5 株（8.3%）分离株分别呈 1 类和 2 类整合子阳性。 1 类整合子内捕获的抗菌素耐药性基因盒的 DNA 测序结果鉴定出以下基因：二氢叶酸还原酶、dfrA1、dfrA5、dfrA7、dfrA12、dfrA17 和 dfrA25； [0103] 氨基糖苷腺苷转移酶、aadA1、aadA2、aadA5、aadA12 和 aadB；氨基糖苷乙酰转移酶，aac(6')-Ib；和氯霉素抗性基因，cmlA1。在 25 个（41.7%）分离株中鉴定出 ESBL。鉴定的ESBL为bla(CTX-M-15)、bla(CTX-M-56)、bla(OXA-1)、bla(SHV-1)、bla(SHV-12)、bla(SHV-32)和bla(TEM-1)基因。此外，我们还对质粒介导的喹诺酮抗性基因 aac(6')-Ib-cr 和 qnrB2 进行了表征，分别在七个 (11.7%) 和两个 (3.3%) 分离株中检测到。这项研究在巴勒斯坦医院的革兰氏阴性细菌中鉴定出了多种类型的抗生素抗性基因，其中许多是在中东地区首次报道。
Sixty Gram-negative bacterial isolates were collected from Palestinian hospitals in 2006. Thirty-two (53.3%) isolates showed multidrug resistance phenotypes. PCR and DNA sequencing were used to characterize integrons and antimicrobial resistance genes. PCR screening showed that 19 (31.7%) and five (8.3%) isolates were positive for class 1 and class 2 integrons, respectively. DNA-sequencing results for the captured antimicrobial resistance gene cassettes within class 1 integrons identified the following genes: dihydrofolate reductases, dfrA1, dfrA5, dfrA7, dfrA12, dfrA17 and dfrA25; aminoglycoside adenyl-transferases, aadA1, aadA2, aadA5, aadA12 and aadB; aminoglycoside acetyltransferase, aac(6')-Ib; and chloramphenicol resistance gene, cmlA1. ESBL were identified in 25 (41.7%) isolates. The identified ESBL were bla(CTX-M-15), bla(CTX-M-56), bla(OXA-1), bla(SHV-1), bla(SHV-12), bla(SHV-32) and bla(TEM-1) genes. Moreover, we characterized the plasmid-mediated quinolone resistance genes, aac(6')-Ib-cr and qnrB2, which were detected in seven (11.7%) and two (3.3%) isolates, respectively. In this study various types of antibiotic resistance genes have been identified in Gram-negative bacteria from Palestinian hospitals, many of which are reported in the Middle East area for the first time.