ANTI-FAS MONOCLONAL-ANTIBODY IS CYTOCIDAL TO HUMAN IMMUNODEFICIENCY VIRUS-INFECTED CELLS WITHOUT AUGMENTING VIRAL REPLICATION
ANTI-FAS MONOCLONAL-ANTIBODY IS CYTOCIDAL TO HUMAN IMMUNODEFICIENCY VIRUS-INFECTED CELLS WITHOUT AUGMENTING VIRAL REPLICATION
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DOI：
10.1073/pnas.87.24.9620
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发表时间：
1990-12-01
影响因子：
11.1
通讯作者：
YONEHARA, S
中科院分区：
文献类型：
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作者：
KOBAYASHI, N;HAMAMOTO, Y;YONEHARA, S
针对与肿瘤坏死因子（TNF）受体相关的200-kDa细胞表面Fas抗原的细胞毒性单克隆抗体（抗Fas mAb）检测了其对人类免疫缺陷病毒（HIV）感染细胞的体外活性。结果表明，TNF和抗Fas单克隆抗体均能选择性地杀伤HIV慢性感染细胞。未感染的细胞对抗体的敏感性低于感染HIV的细胞。当细胞在HIV感染后立即在抗Fas mAb存在下培养时，HIV感染细胞的扩散被抗体抑制。TNF增强了HIV感染细胞中HIV特异性mRNA的合成和多核巨细胞的形成。与此相反，抗Fas单克隆抗体没有增加HIV复制或增强HIV诱导的合胞体形成。结果表明，抗Fas mAb模拟TNF的杀细胞作用，但不增加HIV复制。
A cytotoxic monoclonal antibody (anti-Fas mAb) against the 200-kDa cell surface Fas antigen, which is associated with the tumor necrosis factor (TNF) receptor, was examined for its in vitro activity on human immunodeficiency virus (HIV)-infected cells. It was found that both TNF and anti-Fas mAb selectively killed the chronically HIV-infected cells. Uninfected cells were less sensitive to the antibody than those infected with HIV. When the cells were cultured in the presence of anti-Fas mAb immediately after the HIV infection, the spread of HIV-infected cells was suppressed by the antibody. TNF augmented both the synthesis of HIV-specific mRNA in HIV-infected cells and formation of multinucleated giant cells. In contrast, the anti-Fas mAb did not augment HIV replication or enhance the HIV-induced formation of syncytia. The results indicated that anti-Fas mAb mimicks the cytocidal action of TNF but does not augment HIV replication.