Determination of storage stability of the crude extracts of Ganoderma lucidum using FTIR and 2D-IR spectroscopy
Determination of storage stability of the crude extracts of Ganoderma lucidum using FTIR and 2D-IR spectroscopy
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DOI：
10.1016/j.vibspec.2011.05.008
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发表时间：
2011-09-16
影响因子：
2.5
通讯作者：
Tao, Jia-Xun
中科院分区：
文献类型：
--
作者：
Choong, Yew Keong;Sun, Su-Qin;Tao, Jia-Xun
本文首次探讨并论证了利用FTIR光谱及其相应的二阶导数光谱结合2D-IR光谱分析不同贮藏期灵芝水提物的可行性和优势。为了比较4个贮存期的二阶导数显微指纹光谱，由于样品和标准品中都存在淀粉的检测，所以必须忽略1200 ~ 500 cm的范围。这是至关重要的，因为多糖的主要特征带被分配在这个范围内。淀粉作为外组分的加入被认为是对多糖含量的破坏。事实上，灵芝中的多糖含量对其抗癌特性起主要作用。对1480 ~ 1200 cm（-1）和1700 ~ 1480 cm（-1）进行了解译和直接比较。对于范围（从1800到400厘米（-1）），22个月的样本最接近对照组，其次是38个月、13个月和6个月的产品。提取物的光谱与对照物的匹配不一致，这取决于选择的光谱范围。可以考虑许多因素，这些因素可能会影响对不同储存时间的提取物成分的推断。爱思唯尔B.V.版权所有
In this paper, the feasibility and advantage of employing FTIR spectroscopy and the corresponding second-derivative spectroscopy combined with 2D-IR spectroscopy for the analysis of water extracts of Ganodenno lucidum with different storage durations were investigated and demonstrated for the first time. In order to compare the second-derivative microscopic fingerprint spectra from the four periods of storage, the range from 1200 to 500 cm has to be ignored due to the detection of starch in the samples and the standard as well. This is crucial because the main characteristic band of polysaccharide was assigned within this range. The addition of starch as outer constituent was considered spoilage of polysaccharide content investigation. In fact, polysaccharide content in G. lucidum plays a main role as anti-cancer properties. The ranges from 1480 to 1200 cm(-1) and from 1700 to 1480 cm(-1) were interpreted and directly compared. For the range (from 1800 to 400 cm(-1)), the 22 months sample was closest to the control, followed by 38 months, 13 months and 6 months product. The matching of the extract spectra with the control was not consistent and depended on which range of spectra had been chosen. Many factors can be considered which may have possibly affected inferences of the composition of the extract from different storage durations. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.