The quantitative measurement of DNA hybridization from renaturation rates.

The quantitative measurement of DNA hybridization from renaturation rates.
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DOI:
10.1111/j.1432-1033.1970.tb00830.x
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发表时间:
1970
期刊:
European journal of biochemistry
影响因子:
--
通讯作者:
J. Ley;H. Cattoir;A. Reynaerts
J. Ley;H. Cattoir;A. Reynaerts
中科院分区:
其他
文献类型:
--
作者:
J. Ley;H. Cattoir;A. Reynaerts

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提出了一种基于DNA类型及其混合物的复性率测定来测量细菌之间相关性的新方法。的方程,与复性率的结合程度,计算了几种情况下。给出了测量的最佳条件。该方法从几个方面进行了检验。总结了其优点。
A new method is proposed to measure relatedness amongst bacteria, based on renaturation rate determinations of DNA types and their mixture. The equations, relating the degree of binding with renaturation rates, are calculated for several cases. The optimal conditions for measurements are given. The method was checked in several ways. The advantages are summarized.