The quantitative measurement of DNA hybridization from renaturation rates.
The quantitative measurement of DNA hybridization from renaturation rates.
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DOI:
10.1111/j.1432-1033.1970.tb00830.x
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发表时间:
1970
期刊:
影响因子:
--
通讯作者:
J. Ley;H. Cattoir;A. Reynaerts
中科院分区:
文献类型:
--
作者:
J. Ley;H. Cattoir;A. Reynaerts
A new method is proposed to measure relatedness amongst bacteria, based on renaturation rate determinations of DNA types and their mixture. The equations, relating the degree of binding with renaturation rates, are calculated for several cases. The optimal conditions for measurements are given. The method was checked in several ways. The advantages are summarized.