Oxygen-reactive metabolites are not detected at the effector-target interface during natural killing.
Oxygen-reactive metabolites are not detected at the effector-target interface during natural killing.
复制标题
在自然杀伤过程中，效应器-靶标界面上未检测到氧反应性代谢物。
DOI：
10.1002/jlb.39.5.547
复制
发表时间：
1986
影响因子：
5.5
通讯作者：
Dawson,JR
中科院分区：
文献类型：
--
作者：
Storkus,WJ;Dawson,JR
在自然杀伤（NK）-靶细胞界面未检测到与其他效应-靶细胞相互作用相当的氧反应性代谢物。描述了一种新的鲁米诺包被靶细胞化学发光测定法，其中鲁米诺与双功能交联剂3，3 ′-二硫代双（丙酸N-羟基琥珀酰亚胺酯）（DSP）结合到靶细胞表面。这种基本化学发光测定法的修改排除了检测系统从紧密封闭的细胞间连接，在以前的调查中可能存在的缺陷。鲁米诺结合不影响NK介导的结合物形成或细胞溶解。随着NK活性通过标准系列效应子分级分离程序富集，针对标记的靶细胞产生的化学发光减少。在MO 2+细胞的抗体和补体消耗后，最高NK活性效应子部分中的残留化学发光被消除。这表明单核细胞污染是鲁米诺可检测的氧反应性代谢产物的来源。
Oxygen‐reactive metabolites are not detected at the natural killer (NK)‐target cell interface in quantities comparable to those seen for other effector‐target cell interactions. A novel luminol‐coated target cell chemiluminescence assay is described in which luminol is conjugated to the target cell surface with the bifunctional crosslinker 3,3′‐dithiobis(propionic acid N‐hydroxysuccinimide ester) (DSP). This modification of the basic chemiluminescence assay precludes exclusion of the detection system from the tightly occluded intercellular junction, a possible deficiency in previous investigations. Luminol conjugation does not affect NK‐mediated conjugate formation or cytolysis. As NK activity is enriched by a standard series of effector fractionation procedures, chemiluminescence generated against labeled target cells diminishes. Residual chemiluminescence in the most highly NK‐active effector fraction is ablated upon antibody and complement depletion of MO2+cells. This indicates that monocyte contamination is the source of luminol‐detectable oxygen‐reactive metabolites.