A multiplex-PCR to identify hepatitis B virus -: genotypes A-F
A multiplex-PCR to identify hepatitis B virus -: genotypes A-F
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DOI：
10.1016/s1386-6532(03)00084-2
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发表时间：
2004-01-01
影响因子：
8.8
通讯作者：
Schaefer, S
中科院分区：
文献类型：
--
作者：
Kirschberg, O;Schüttler, C;Schaefer, S
乙型肝炎病毒 (HBV) 已知有 8 种基因型 (A-H)，其核苷酸序列变异大于 8%。最近的几篇出版物发现，抗病毒治疗的临床过程和结果取决于感染乙肝病毒株的基因型。大型流行病学研究需要一个快速、可靠且可以对大量样本进行分析的系统。我们开发了一种多重 PCR 检测方法，使用针对 HBV 基因型 A-F 的基因型特异性引物对。这些引物对在单一或多重 PCR 中特异性扩增各自基因型的 HBV DNA。该测定的灵敏度在 10(4) 基因组当量范围内。 (C) 2003 Elsevier B.V. 保留所有权利。
Eight genotypes (A-H) of hepatitis B virus (HBV) are known with variations in nucleotide sequences greater than 8%. Several recent publications found that the clinical course and outcome of antiviral therapy depended on the genotype of the infecting HBV strain. Large epidemiological studies will require the availability of a system which is rapid, reliable and can be performed on a large number of samples. We have developed a multiplex-PCR assay which uses genotype-specific primer pairs for HBV genotypes A-F. These primer pairs specifically amplified HBV DNA of the respective genotype, either in single or in multiplex-PCR. Sensitivity of the assay was in the range of 10(4) genome equivalents. (C) 2003 Elsevier B.V. All rights reserved.