Changes in electrophysiological properties of cat hypoglossal motoneurons during carbachol-induced motor inhibition
Changes in electrophysiological properties of cat hypoglossal motoneurons during carbachol-induced motor inhibition
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DOI：
10.1016/s0006-8993(00)02955-3
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发表时间：
2000-12-08
期刊：
影响因子：
2.9
通讯作者：
Chase, MH
中科院分区：
文献类型：
--
作者：
Fung, SJ;Yamuy, J;Chase, MH
从基础科学的角度来看，睡眠过程中舌下运动神经元的控制是很重要的，也有助于理解导致阻塞性睡眠呼吸暂停综合征的咽阻塞的基础。在本研究中，我们使用细胞内记录技术来确定成年猫的膜特性的变化，在这种情况下，通过向桥状被体注射卡巴考（as -卡巴考）产生张力。在AS-carbachol期间，86%记录的舌下运动神经元的电学性质分析发现其突触后被抑制；其余14%的电学性质与控制条件下记录的运动神经元相似。这些细胞的电学特性发生了变化。在as期间，氨基苯酚也表现出大振幅的抑制性突触电位。在坐骨神经刺激后，在控制条件下以去极化电位反应的下腭运动神经元在as -carbachol期间表现出超极化电位，在as -carbachol期间记录的自发电位和诱发抑制电位与之前在类似实验条件下以及在自然发生的活跃睡眠中观察到的三叉神经和脊髓运动神经元的自发电位和诱发抑制电位相当。基于我们在三室神经元模型中发现的输入电阻和膜时间常数变化的建模计算表明，舌下运动神经元模型的所有三个室都存在分流。综上所述，这些数据表明突触后抑制驱动广泛分布于AS-carbachol期间舌下运动神经元的体-树突树。这些突触后抑制作用可能与阻塞性睡眠呼吸暂停的病理生理有关。(C) 2000 Elsevier Science B.V.版权所有
The control of hypoglossal motoneurons during sleep is important from a basic science perspective as well as to understand the bases for pharyngeal occlusion which results in the obstructive sleep apnea syndrome, In the present work, we used intracellular recording techniques to determine changes in membrane properties in adult cats in which atonia was produced by the injection of carbachol into the pontine tegmentum (AS-carbachol). During AS-carbachol, 86% of the recorded hypoglossal motoneurons were found to be postsynaptically inhibited on the basis of analyses of their electrical properties; the electrical properties of the remaining 14% were similar to motoneurons recorded during control conditions. Those cells that exhibited changes in their electrical properties. during AS-carbachol also displayed large-amplitude inhibitory synaptic potentials. Following sciatic nerve stimulation, hypoglossal motoneurons which responded with a depolarizing potential during control conditions exhibited a hyperpolarizing potential during AS-carbachol, Both spontaneous and evoked inhibitory potentials recorded during AS-carbachol were comparable to those that have been previously observed in trigeminal and spinal cord motoneurons under similar experimental conditions as well as during naturally occurring active sleep. Calculations based on modeling the changes that we found in input resistance and membrane time constant with a three-compartment neuron model suggest that shunts are present in all three compartments of the hypoglossal motoneuron model. Taken together, these data indicate that postsynaptic inhibitory drives an widely distributed on the soma-dendritic tree of hypoglossal motoneurons during AS-carbachol. These postsynaptic inhibitory actions are likely to be involved in the pathophysiology of obstructive sleep apnea. (C) 2000 Elsevier Science B.V. All rights reserved.