Stimulation of protein phosphorylation in Swiss mouse 3T3 cells by human platelet derived growth factor.
Stimulation of protein phosphorylation in Swiss mouse 3T3 cells by human platelet derived growth factor.
复制标题
人血小板衍生生长因子刺激瑞士小鼠 3T3 细胞中的蛋白质磷酸化。
DOI：
10.1016/0006-291x(81)91700-9
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发表时间：
1981
影响因子：
3.1
通讯作者：
Deuel,TF
中科院分区：
文献类型：
--
作者：
Nishimura,J;Deuel,TF
纯化的人血小板衍生生长因子刺激1033，000 M.W. Swiss小鼠3 T3细胞中的蛋白质。PDGF刺激32 P标记的细胞3 min后可检测到磷酸化，10 min后标记，PDGF磷酸化的S_（0.5）为3 ng/ml。在这1033，000 M.W.的水解产物中鉴定出磷酸丝氨酸，而非磷酸酪氨酸。蛋白
Purified human platelet derived growth factor stimulates the phosphorylation of an ⋍33,000 M.W. protein in Swiss mouse 3T3 cells. Phosphorylation can be detected 3 min after32P-labelled cells are stimulated by PDGF and is marked after 10 min. The S0.5of PDGF for phosphorylation is 3 ng/ml. Phosphoserine but not phosphotyrosine has been identified in hydrolysates of this ⋍33,000 M.W. protein.