The Efficiency of Bone Marrow Aspiration for the Harvest of Connective Tissue Progenitors from the Human Iliac Crest
The Efficiency of Bone Marrow Aspiration for the Harvest of Connective Tissue Progenitors from the Human Iliac Crest
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DOI：
10.2106/jbjs.17.00094
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发表时间：
2017-10-04
影响因子：
5.3
通讯作者：
Muschler, George F.
中科院分区：
文献类型：
--
作者：
Patterson, Thomas E.;Boehm, Cynthia;Muschler, George F.
背景资料：合理的设计和优化的组织工程策略，以细胞为基础的治疗需要一个基线的浓度和患病率的成骨祖细胞群体在源组织的理解。本研究的目的是（1）确定骨髓抽吸作为成骨结缔组织祖细胞（CTP-O）收获的一种手段与从髂骨松质骨收获相比的效率和个体之间的差异，以及（2）确定CTP-O在天然松质骨中的位置及其在骨髓空间和小梁表面组织室之间的分布。方法：从33名人类受试者的前髂嵴获取了8份2 mL骨髓抽吸（BMA）样本和1份7 mm经皮质活检样本。从髂骨松质骨（ICB）样本中获得两个细胞群。将ICB样品置于含有抗真菌药的aMEM（α-最小必需培养基）中，并用锋利的骨凿切成小块（直径1 - 2 mm）。可从ICB样品机械解离的细胞被定义为骨髓腔（IC-MS）细胞，仅在酶消化后解离的细胞被定义为小梁表面（IC-TS）细胞。3个来源的骨和骨髓来源的细胞进行了比较的基础上的细胞性和浓度和患病率的CTP-O通过菌落形成单位（CFU）analysis.Results：大的变化被认为是患者之间的细胞和CTP-O产量从IC-MS，IC-TS，和BMA样品和在IC-MS和IC-TS馏分之间的CTP-O的相对分布。CTP-O流行率在IC-TS组分中最高，是IC-MS组分的11.4倍（p < 0.0001），是BMA组分的1.7倍。然而，ICB中CTP-Os的中位浓度（结合MS和TS组分）仅为BMA中CTP-Os的3.04 +/- 1.1倍（4，265与1，402 CTP/mL相比; p = 0.00004）。结论：在给定的针头部位进行2 mL体积的骨髓抽吸是收获CTP-Os的有效方法，尽管用外周血稀释。然而，CTP-Os的中位浓度比自体髂骨松质骨低3倍。CTP-O在IC-MS和IC-TS组分之间的分布在患者中差异很大。
Background: The rational design and optimization of tissue engineering strategies for cell-based therapy requires a baseline understanding of the concentration and prevalence of osteogenic progenitor cell populations in the source tissues. The aim of this study was to (1) define the efficiency of, and variation among individuals in, bone marrow aspiration as a means of osteogenic connective tissue progenitor (CTP-O) harvest compared with harvest from iliac cancellous bone, and (2) determine the location of CTP-Os within native cancellous bone and their distribution between the marrow-space and trabecular-surface tissue compartments.Methods: Eight 2-mL bone marrow aspiration (BMA) samples and one 7-mm transcortical biopsy sample were obtained from the anterior iliac crest of 33 human subjects. Two cell populations were obtained from the iliac cancellous bone (ICB) sample. The ICB sample was placed into aMEM (alpha-minimal essential medium) with antibiotic-antimycotic and minced into small pieces (1 to 2 mm in diameter) with a sharp osteotome. Cells that could be mechanically disassociated from the ICB sample were defined as marrow-space (IC-MS) cells, and cells that were disassociated only after enzymatic digestion were defined as trabecular-surface (IC-TS) cells. The 3 sources of bone and marrow-derived cells were compared on the basis of cellularity and the concentration and prevalence of CTP-Os through colony-forming unit (CFU) analysis.Results: Large variation was seen among patients with respect to cell and CTP-O yield from the IC-MS, IC-TS, and BMA samples and in the relative distribution of CTP-Os between the IC-MS and IC-TS fractions. The CTP-O prevalence was highest in the IC-TS fraction, which was 11.4-fold greater than in the IC-MS fraction (p < 0.0001) and 1.7-fold greater than in the BMA fraction. However, the median concentration of CTP-Os in the ICB (combining MS and TS fractions) was only 3.04 +/- 1.1-fold greater than that in BMA (4,265 compared with 1,402 CTP/mL; p = 0.00004).Conclusions: Bone marrow aspiration of a 2-mL volume at a given needle site is an effective means of harvesting CTP-Os, albeit diluted with peripheral blood. However, the median concentration of CTP-Os is 3-fold less than from native iliac cancellous bone. The distribution of CTP-Os between the IC-MS and IC-TS fractions varies widely among patients.