Watermelon chlorotic stunt virus from the Sudan and Iran:: Sequence comparisons and identification of a whitefly-transmission determinant
Watermelon chlorotic stunt virus from the Sudan and Iran:: Sequence comparisons and identification of a whitefly-transmission determinant
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DOI：
10.1094/phyto.2000.90.6.629
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发表时间：
2000-06-01
期刊：
影响因子：
3.2
通讯作者：
Gronenborn, B
中科院分区：
文献类型：
--
作者：
Kheyr-Pour, A;Bananej, K;Gronenborn, B
对两个西瓜褪绿矮化病毒(WmCSV)苏丹分离物和伊朗分离物的基因组进行了克隆和测序。与其他双生病毒的序列关系表明，WmCSV是一种典型的东半球双生病毒，具有两部分基因组。来自非洲和中东的两个地理位置遥远的WmCSV分离株有非常高的总体序列相似性：它们的DNA-A和DNA-B组分之间的序列相似性分别为98%和96%，并且它们各自的衣壳蛋白是相同的。衣壳蛋白(N131D)的单一氨基酸变化使WmCSV粉虱不能传播。衣壳的这一区域也与烟粉虱传播番茄黄化卷叶病毒有关。
The genomes of two Watermelon chlorotic stunt virus (WmCSV) isolates, one from the Sudan and one from Iran, were cloned and sequenced. Sequence relationship with other geminiviruses characterizes WmCSV as a typical Eastern Hemisphere geminivirus with a bipartite genome. The two geographically distant WmCSV isolates from Africa and the Middle East share a very high overall sequence similarity: 98% between their DNA-A and 96% between their DNA-B components, and their respective capsid proteins are identical. A single amino acid change in the capsid protein (N131D) renders WmCSV whitefly nontransmissible. This region of the capsid is also implicated in transmission by Bemisia tabaci of Tomato yellow leaf curl virus.