Characterization of H5N1 Highly Pathogenic Avian Influenza Virus Isolated from a Mountain Hawk Eagle in Japan
Characterization of H5N1 Highly Pathogenic Avian Influenza Virus Isolated from a Mountain Hawk Eagle in Japan
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DOI：
10.1292/jvms.09-0478
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发表时间：
2010-04-01
影响因子：
1.2
通讯作者：
Ito, Toshihiro
中科院分区：
文献类型：
--
作者：
Shivakoti, Sakar;Ito, Hiroshi;Ito, Toshihiro
2007年1月4日，在日本熊本县发现了一只瘦弱的山鹰。从死禽气管和泄殖腔拭子中分离到H5 N1亚型高致病性禽流感病毒。1月13日，宫崎县一家养鸡场爆发了由H5 N1毒株引起的高致病性禽流感。三周内。另外发生了三起疫情（两起在宫崎县，一起在冈山县）。为探讨鹰禽分离物与鸡分离物的亲缘关系，我们对鹰禽分离物的生长、致病性和系统发育进行了研究。病毒滴度最高的是脑（10（7.25）EID 50/g），其次是气管和肌肉（分别为10（2.65）和10（2.50）EID 50/g）。在血凝素（HA）切割位点的序列分析显示，该分离物的典型的毒力型序列。R-R-R-K-K-R系统进化分析表明，该病毒属于青海湖型病毒组。HA基因的同源性检索也显示与2007年的宫崎和冈山分离株以及2006年的韩国分离株具有主要相似性（超过99%）。这些结果表明，青海湖型H5 N1高致病性禽流感病毒是新近从亚洲大陆传入日本的，并已于2007年1月初在九州地区的自然环境中存在。
On January 4, 2007, an emaciated mountain hawk-eagle was found in Kumamoto Prefecture, Japan. Highly pathogenic avian influenza (HPAI) virus subtype H5N1 was isolated from both tracheal and cloacal swabs of the dead bird. On January 13, an outbreak of HPAI, caused by H5N1 strain, occurred in a chicken farm in Miyazaki Prefecture. Within three weeks. three additional outbreaks had occurred (two in Miyazaki Prefecture and one in Okayama Prefecture). To investigate the relationship between the hawk-eagle isolate and chicken isolates, we studied the virus growth, pathogenicity, and phylogenetic information of this hawk-eagle isolate. The highest virus titer was found in the brain (10(7.25) EID50/g), followed by trachea and muscle (10(2.65) and 10(2.50) EID50/g, respectively). Sequence analysis at the hemagglutinin (HA) cleavage site of this isolate revealed a typical virulent-type sequence. R-R-R-K-K-R. Phylogenetic analysis demonstrated that the hawk-eagle isolate belongs to Qinghai Lake type virus group. A homology search of the HA gene also showed major similarity (more than 99%) to the Miyazaki and Okayama isolates in 2007 and also Korean isolates in 2006. These results suggest that Qinghai Lake type H5N1 HPAI virus was newly introduced from Asian Continent into Japan, and had already present in natural environment of Kyusyu district in the beginning of January 2007.