Isolation and characterization of 16 microsatellite loci in the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae: Scolytinae)
Isolation and characterization of 16 microsatellite loci in the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae: Scolytinae)
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DOI：
10.1111/j.1755-0998.2009.02579.x
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发表时间：
2009-05-01
影响因子：
7.7
通讯作者：
Cooke, J. E. K.
中科院分区：
文献类型：
--
作者：
Davis, C. S.;Mock, K. E.;Cooke, J. E. K.
从山松甲虫（Dendroctonus ponderosae Hopkins）中分离出16个多态微卫星位点，并建立了4个多重反应扩增条件。在两个样本群体中，每个位点检测到3 ~ 14个等位基因。观察到的杂合度和预期杂合度分别为0.000 ~ 0.902和0.100 ~ 0.830。在一个样本群体中有三个位点偏离了Hardy-Weinberg平衡。其中一个基因座可能与性别有关。这些标记将有助于研究这一重要害虫的种群结构。
We isolated 16 polymorphic microsatellite loci in the mountain pine beetle (Dendroctonus ponderosae Hopkins) and developed conditions for amplifying these markers in four multiplex reactions. Three to 14 alleles were detected per locus across two sampled populations. Observed and expected heterozygosities ranged from 0.000 to 0.902 and from 0.100 to 0.830, respectively. Three loci deviated from Hardy-Weinberg equilibrium in one sampled population. One of these loci may be sex linked. These markers will be useful in the study of population structure in this important pest species.