Extracellular matrix changes in human corneas after radial keratotomy
Extracellular matrix changes in human corneas after radial keratotomy
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DOI：
10.1006/exer.1998.0511
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发表时间：
1998-09-01
影响因子：
3.4
通讯作者：
Maguen, E
中科院分区：
文献类型：
--
作者：
Ljubimov, AV;Alba, SA;Maguen, E
放射状角膜切开术后，人角膜细胞外基质和基底膜发生改变。10个正常的和5个放射状角膜切开术尸检角膜（2在术后6个月，3在术后3年）进行了研究，免疫荧光抗体28细胞外基质和基底膜成分。在放射状角膜切开术瘢痕外，研究的成分呈正态分布。间质细胞外基质中，只有III型胶原聚集在周围。伤疤上皮栓周围的基底膜除TV型胶原外，其余成分正常。它的α 1-α 2链，通常只存在于角膜缘基底膜，出现在所有的插头。在这些区域中α 3和α 4链非常弱或不存在，与非瘢痕区域相反。这种基底膜图案类似于正常角膜缘，而不是中央角膜图案。角蛋白3也有一个边缘样，基底上的表达在塞子上皮。正常角膜基底膜和细胞外基质中均无tenascin-C、tenascin-1、Vm型胶原和XIV型胶原的存在。只有腱生蛋白-C在术后3年的前部瘢痕中显示出比术后6个月更少的染色，但在后部瘢痕中仍然持续存在。即使在放射状角膜切开术后3年，瘢痕愈合不完全也很明显。它表现为异常细胞外基质的积累，在前面和后面的疤痕和边缘型IV型胶原亚型的基底膜周围的上皮栓。(C)北京：科学出版社.
Extracellular matrix and basement membrane alterations were identified in human corneas after radial keratotomy. Ten normal and five radial keratotomy autopsy corneas (two at 6 months post surgery, and three at 3 years post surgery) were studied by immunofluorescence with antibodies to 28 extracellular matrix and basement membrane components. Outside of radial keratotomy scars, an studied components had a normal distribution. Of stromal extracellular matrix, only type III collagen accumulated around the. scars. The basement membrane around epithelial plugs had a normal composition except for type TV collagen. Its alpha 1-alpha 2 chains, normally present only in the limbal basement membrane, appeared around all plugs. alpha 3 and alpha 4 chains were very weak or absent in these areas, contrary to nonscarred areas. This basement membrane pattern was similar to the normal limbal but not to the central corneal pattern. Keratin 3 also had a limbal-like, suprabasal expression in the plug epithelium. The stroma around the scars accumulated tenascin-C, fibrillin-1, types Vm and XIV collagen, all of which were absent from normal corneal basement membrane and extracellular matrix. Only tenascin-C showed less staining in anterior scars 3 years post surgery than 6 months post surgery, but still persisted in posterior scars. Incomplete scar healing was evident even 3 years post radial keratotomy. It was manifested by the accumulation of abnormal extracellular matrix in the anterior and posterior scars and by the limbal-lilte pattern of type IV collagen isoforms in the basement membrane around epithelial plugs. (C) 1998 Academic Press.