Impact of dietary propionate on fructose-induced changes in lipid metabolism, gut microbiota and short-chain fatty acids in mice
Impact of dietary propionate on fructose-induced changes in lipid metabolism, gut microbiota and short-chain fatty acids in mice
复制标题
DOI：
10.1080/09637486.2020.1773415
复制
发表时间：
2020-06-03
影响因子：
3.9
通讯作者：
Stangl, Gabriele I.
中科院分区：
文献类型：
--
作者：
Bruetting, Christine;Lara Bisch, Milena;Stangl, Gabriele I.
丙酸具有抗菌活性，被认为是影响脂代谢的物质。在这里，我们研究了丙酸对果糖喂养小鼠的脂肪代谢和肠道微生物群的影响，作为饮食诱导的脂肪变性和肠道微生物失调的模型。因此，48只雄性野生型小鼠在等能饲料中添加0%果糖(F-)或40%果糖(F+)，其中含有0%丙酸(P-)或1%丙酸(P+)，连续7周。接受F+饮食的小鼠出现脂肪肝，小肠蛋白细菌和结肠放线菌较少，并以细菌属(如Allobaculum、Lachnospiraceae和Escherichia)的变化为特征。有趣的是，饲喂F+的小鼠血液中丙酸和丁酸的水平高于饲喂F的小鼠(p<；0.05)。丙酸治疗既不影响肝脏或血浆脂类，也不影响循环中的SCFAs水平。除韦氏杆菌外，其他细菌门均不受丙酸影响。
Propionate has antimicrobial activity and is suggested to influence lipid metabolism. Here, we investigated the effect of propionate on lipid metabolism and the gut microbiome in fructose-fed mice as a model of diet-induced steatosis and gut dysbiosis. Therefore, 48 male wild-type mice were fed isoenergetic diets with either 0% fructose (F-) or 40% fructose (F+) that contained 0% propionate (P-) or 1% propionate (P+) for 7 weeks. Mice that received the F+ diets developed fatty livers, had fewer small intestinal proteobacteria and colonic actinobacteria and were characterised by changes in bacterial genera (e.g.,Allobaculum,Lachnospiraceae, andEscherichia). Interestingly, mice fed the F+ diets had higher levels of propionate and butyrate in the circulation than mice fed the F- diets (p < 0.05). Treatment with propionate influenced neither hepatic or plasma lipids nor levels of circulating SCFAs. With the exception ofVerrucomicrobia, other bacterial phyla were not affected by propionate.