Thrombolytic enhancement with perfluorocarbon-exposed sonicated dextrose albumin microbubbles
Thrombolytic enhancement with perfluorocarbon-exposed sonicated dextrose albumin microbubbles
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DOI：
10.1016/s0002-8703(96)90006-x
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发表时间：
1996-11-01
影响因子：
4.8
通讯作者：
Xie, F
中科院分区：
文献类型：
--
作者：
Porter, TR;LeVeen, RF;Xie, F
虽然单独使用低强度高频超声（US）似乎能引起最小的溶栓，但US联合充气微泡确实能增加尿激酶（UK）介导的凝块溶解（CL）的量。由于这种现象可能是由空化诱导的流介导的，我们假设全氟化碳暴露的超声葡萄糖白蛋白（PESDA）微泡比充气微泡更稳定，也可能增强US诱导的溶栓。我们测量了同样大小的血栓（1.0 +/- 0.1 mg）的百分比CL，这些血栓由新鲜抽取的血液孵育2小时，然后暴露于20 kHz US （0.846 MPa峰值负压）。将血栓浸泡在4ml生理盐水溶液中，单独使用UK (20,000 U)，单独使用PESDA或PESDA与UK联合使用。使用PESDA和治疗US获得的CL百分比也与室内充气超声葡萄糖白蛋白（RASDA）微泡获得的CL百分比进行了比较。与US单独（24% +/- 13% CL）或UK单独（17% +/- 3% CL）相比，PESDA联合US产生的CL明显更好（43% +/- 17%,p < 0.05）， PESDA联合US产生的CL也明显高于RASDA联合US （28% +/- 9%, p < 0.05）。PESDA + UK + US联合使用可获得最佳CL （60% +/- 14% CL）。我们的结论是，PESDA微泡在低频换能器的干扰下可能能够诱导溶栓。
Whereas low-intensity, high-frequency ultrasound (US) alone appears to cause minimal thrombolysis, US combined with air-filled microbubbles does increase the amount of urokinase (UK)-mediated clot lysis (CL), Because this phenomenon may be mediated by cavitation-induced streaming, we hypothesized that perfluorocarbon-exposed sonicated dextrose albumin (PESDA) microbubbles, which are more stable than air-filled microbubbles, may also enhance US-induced thrombolysis, We measured the percentage CL of equally sized thrombi (1.0 +/- 0.1 mg) made from freshly drawn blood incubated for 2 hours and then exposed to 20 kHz US (0.846 MPa peak negative pressure). The thrombi were bathed in 4 ml of saline solution, UK alone (20,000 U), PESDA alone, or a combination of PESDA with UK. The percentage CL achieved with PESDA and therapeutic US was also compared with the percentage CL achieved with room air-filled sonicated dextrose albumin (RASDA) microbubbles. When compared with US alone (24% +/- 13% CL) or UK alone (17% +/- 3% CL), PESDA plus US produced significantly better CL (43% +/- 17%; p < 0.05), PESDA combined with US also produced significantly greater CL than RASDA combined with US (28% +/- 9%; p < 0.05). The optimal CL was achieved with a combination of PESDA with UK with US (60% +/- 14% CL). We conclude that PESDA microbubbles alone may be capable of inducing thrombolysis when insonified with a low-frequency transducer.