Maternal low-protein diet induces changes in the cardiovascular autonomic modulation in male rat offspring
Maternal low-protein diet induces changes in the cardiovascular autonomic modulation in male rat offspring
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DOI：
10.1016/j.numecd.2014.07.011
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发表时间：
2015-01-01
影响因子：
3.9
通讯作者：
Costa-Silva, J. H.
中科院分区：
文献类型：
--
作者：
Barros, M. A. V.;de Brito Alves, J. L.;Costa-Silva, J. H.
背景和目的：母亲营养不良可导致动脉高血压的发生。我们调查的影响，母亲的低蛋白饮食对心血管自主控制的后代。方法和结果：雄性Wistar大鼠分为两组，根据他们的母亲在妊娠期和哺乳期的饮食：对照组（正常蛋白，NP，17%酪蛋白; n=14）和低蛋白（LP，8%酪蛋白; n=14）组。直接测量动脉压（AP）记录从清醒的90天大的雄性后代。LP后代表现出比NP大鼠更高的平均AP（NP：93 +/-4 vs. LP：113 +/-2 mmHg; p < 0.05），而心率（HR）在两组中相似。在频谱分析中，LP组在低（NP：1.98 +/- 0.25 vs. LP：3.7 +/- 0.3 mmHg（2）; p < 0.05）和高（NP：1.28 +/- 0.18 vs. LP：2.13 +/- 0.42 mmHg（2）; p < 0.05）收缩期动脉压（SAP）频率处显示出更高的功率。在脉冲间隔中，LP组的LF/HF比值增加（NP：0.32 vs. LP：0.56; p < 0.05）。普萘洛尔（4 mg/kg，静脉（iv））给药后，LP组的心动过缓更高（NP：-36 +/-8 vs. LP：-94 +/-12 bpm; p < 0.05），甲基阿托品（2 mg/kg，iv）给药后，心动过速与NP组相似。在给予神经节阻滞剂（六烃季铵; 25 mg/kg，iv）后，LP动物在AP中显示出更大的Δ变化（NP：-33.7 +/-5 vs. LP：-53.6 +/-4 mmHg; p < 0.05）。结论：经历蛋白质营养不良的大鼠表现出心血管交感神经张力的增加，这有助于在这些动物中观察到的AP升高。(C)2014爱思唯尔有限公司版权所有。
Background and aims: Maternal undernutrition induces development of the arterial hypertension. We investigated the effects of a maternal low-protein diet on cardiovascular autonomic control in the offspring.Methods and results: Male Wistar rats were divided into two groups according to the diets of their mothers during gestation and lactation: the control (normal protein, NP, 17% casein; n=14) and low-protein (LP, 8% casein; n=14) groups. Direct measurements of arterial pressure (AP) were recorded from wakeful 90-day-old male offspring. The LP offspring presented higher mean AP than did the NP rats (NP: 93 +/- 4 vs. LP: 113 +/- 2 mmHg; p < 0.05), whereas the heart rate (HR) was similar in the two groups. In the spectral analysis, the LP group showed higher power at low (NP: 1.98 +/- 0.25 vs. LP: 3.7 +/- 0.3 mmHg(2); p < 0.05) and high (NP: 1.28 +/- 0.18 vs. LP: 2.13 +/- 0.42 mmHg(2); p < 0.05) frequencies of systolic arterial pressure (SAP). In the pulse interval, the LP group presented an increase in the LF/HF ratio (NP: 0.32 vs. LP: 0.56; p < 0.05). After propranolol (4 mg/kg, intravenous (iv)), the bradycardia was higher in the LP group (NP: -36 +/- 8 vs. LP: -94 +/- 12 bpm; p < 0.05), after methylatropine (2 mg/kg, iv), the tachycardia was similar to NP group. After administration of the ganglionic blocker (hexamethonium; 25 mg/kg, iv), the LP animals showed larger delta variation in the AP (NP: -33.7 +/- 5 vs. LP: -53.6 +/- 4 mmHg; p < 0.05).Conclusion: The rats subjected to protein malnutrition presented an increase in the cardiovascular sympathetic tone, which contributed to the elevated AP observed in these animals. (C) 2014 Elsevier B.V. All rights reserved.