Differential expression of nuclear lamin proteins during chicken development.
Differential expression of nuclear lamin proteins during chicken development.
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鸡发育过程中核层粘连蛋白的差异表达。
DOI：
10.1083/jcb.105.1.577
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发表时间：
1987-07
期刊：
影响因子：
--
通讯作者：
Nigg EA
中科院分区：
文献类型：
--
作者：
Lehner CF;Stick R;Eppenberger HM;Nigg EA
通过免疫细胞化学、定量免疫印迹和二维凝胶电泳等方法，分析了鸡胚发育过程中核层粘胶蛋白的分布。在雌雄种系配子体发生过程中，核膜A、B1和B2的表达模式没有质的差异，但在体细胞组织发育过程中，核膜的组成发生了深刻的变化。最出乎意料的是，早期的鸡胚胎被发现几乎不含层粘连蛋白A，尽管它们含有大量的层粘连蛋白B1和B2。在胚胎发育过程中，层粘连蛋白A在许多组织中变得越来越突出，而层粘连蛋白B1的数量则减少。有趣的是，这些变化的程度和发育时间表现出明显的组织特异性差异。Lamin B2在研究的所有细胞类型中表达量相当恒定（除了粗筋期生殖细胞）。这些结果对所谓的单个层粘连蛋白的功能特化具有启示意义。此外，他们认为核层组成的改变可能对核结构中细胞或组织特异性差异的建立很重要。
By immunocytochemistry, quantitative immunoblotting, and two- dimensional gel electrophoresis, we have analyzed the distribution of nuclear lamin proteins during chicken embryonic development. Whereas no qualitative differences in the patterns of expression of lamins A, B1, and B2 were observed during gametogenesis in either the female or the male germ line, profound changes in the composition of the nuclear lamina occurred during the development of somatic tissues. Most unexpectedly, early chicken embryos were found to contain little if any lamin A, although they contained substantial amounts of lamins B1 and B2. During embryonic development, lamin A became increasingly prominent, whereas the amounts of lamin B1 decreased in many tissues. Interestingly, the extent and the developmental timing of these changes displayed pronounced tissue-specific variations. Lamin B2 was expressed in fairly constant amounts in all cell types investigated (except for pachytene-stage germ cells). These results have implications for the purported functional specializations of individual lamin proteins. In addition, they suggest that alterations in the composition of the nuclear lamina may be important for the establishment of cell- or tissue-specific differences in nuclear architecture.