SPECTROSCOPIC DETERMINATION OF TRYPTOPHAN AND TYROSINE IN PROTEINS
SPECTROSCOPIC DETERMINATION OF TRYPTOPHAN AND TYROSINE IN PROTEINS
复制标题
DOI：
10.1021/bi00859a010
复制
发表时间：
1967-01-01
期刊：
影响因子：
2.9
通讯作者：
EDELHOCH, H
中科院分区：
文献类型：
--
作者：
EDELHOCH, H
提出了一种快速测定蛋白质中色氨酸的方法。它是基于蛋白质在288米和280米[亩]处溶解在6[图像]盐酸胍中的吸光度测量。被封闭的色氨基(N-乙酰基-L-色氨基)和酪氨基(甘氨酰-L-酪氨酸基)被选为模型物质来表示蛋白质中这两个生色团的吸光度。酪氨酸是与色氨酸同时测量的，但在蛋白质中的精确度要低得多，因为蛋白质的大部分吸收是由于色氨酸。基于酪氨酸在碱性条件下的电离，提出了一种独立的酪氨酸分析方法。
A rapid method for the determination of tryptophan in proteins is presented. It is based on absorbance measurements at 288 and 280 m[mu] of the protein dissolved in 6 [image] guanidine hydrochloride. Blocked tryptophanyl (N-acetyl-L-trypto-phanamide) and tyrosyl (glycyl-L-tyrosylglycine) compounds were selected as model substances to represent the absorbance of the 2 chromophores in proteins. Tyrosine is measured simultaneously with tryptophan but with much less precision in proteins where most of the absorpiton is due to tryptophan. An independent procedure of tyrosine analysis is presented also which is based on its ionization in alkali.