CLONING OF AN MYCOBACTERIUM-TUBERCULOSIS DNA FRAGMENT ASSOCIATED WITH ENTRY AND SURVIVAL INSIDE CELLS
CLONING OF AN MYCOBACTERIUM-TUBERCULOSIS DNA FRAGMENT ASSOCIATED WITH ENTRY AND SURVIVAL INSIDE CELLS
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DOI：
10.1126/science.8367727
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发表时间：
1993-09-10
期刊：
影响因子：
56.9
通讯作者：
RILEY, LW
中科院分区：
文献类型：
--
作者：
ARRUDA, S;BOMFIM, G;RILEY, LW
结核分枝杆菌感染了世界上三分之一的人口。这种广泛的感染取决于生物体通过进入巨噬细胞并在其中存活来逃避宿主防御的能力。M.结核病已经被克隆;这些赋予非致病性大肠杆菌菌株侵入HeLa细胞、增强巨噬细胞吞噬作用并在人巨噬细胞内存活至少24小时的能力。这种进入哺乳动物细胞并在巨噬细胞内存活的能力定位于克隆的M.结核病DNA片段。
Mycobacterium tuberculosis infects one-third of the world's human population. This widespread infection depends on the organism's ability to escape host defenses by gaining entry and surviving inside the macrophage. DNA sequences of M. tuberculosis have been cloned; these confer on a nonpathogenic Escherichia coli strain an ability to invade HeLa cells, augment macrophage phagocytosis, and survive for at least 24 hours inside the human macrophage. This capacity to gain entry into mammalian cells and survive inside the macrophage was localized to two distinct loci on the cloned M. tuberculosis DNA fragment.