Toll-like receptor-3 is dispensable for the innate microRNA response to West Nile virus (WNV).
Toll-like receptor-3 is dispensable for the innate microRNA response to West Nile virus (WNV).
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DOI：
10.1371/journal.pone.0104770
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Dittmer DP
中科院分区：
文献类型：
--
作者：
Chugh PE;Damania BA;Dittmer DP
The innate immune response to West Nile virus (WNV) infection involves recognition through toll-like receptors (TLRs) and RIG-I-like receptors (RLRs), leading to establishment of an antiviral state. MiRNAs (miRNAs) have been shown to be reliable biomarkers of TLR activation. Here, we sought to evaluate the contribution of TLR3 and miRNAs to the host response to WNV infection. We first analyzed HEK293-NULL and HEK293-TLR3 cells for changes in the innate immune response to infection. The presence of TLR3 did not seem to affect WNV load, infectivity or phosphorylation of IRF3. Analysis of experimentally validated NFκB-responsive genes revealed a WNV-induced signature largely independent of TLR3. Since miRNAs are involved in viral pathogenesis and the innate response to infection, we sought to identify changes in miRNA expression upon infection in the presence or absence of TLR3. MiRNA profiling revealed 70 miRNAs induced following WNV infection in a TLR3-independent manner. Further analysis of predicted gene targets of WNV signature miRNAs revealed genes highly associated with pathways regulating cell death, viral pathogenesis and immune cell trafficking.
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影响因子：
6.7
作者：
Diamond MS
通讯作者：
Diamond MS
影响因子：
9.3
作者：
Getts DR;Terry RL;Getts MT;Müller M;Rana S;Deffrasnes C;Ashhurst TM;Radford J;Hofer M;Thomas S;Campbell IL;King NJ
通讯作者：
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影响因子：
5.4
作者：
Errett, John S.;Suthar, Mehul S.;Gale, Michael, Jr.
通讯作者：
Gale, Michael, Jr.
影响因子：
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作者：
Chugh, Pauline;Dittmer, Dirk P.
通讯作者：
Dittmer, Dirk P.
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DOI：
10.1073/pnas.0700544104
发表时间：
2007-07-10
影响因子：
11.1
作者：
Diao, Feici;Li, Shu;Shu, Hong-Bing
通讯作者：
Shu, Hong-Bing