Unique hybrids between the fungal pathogens Cryptococcus neoformans and Cryptococcus gattii
Unique hybrids between the fungal pathogens Cryptococcus neoformans and Cryptococcus gattii
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DOI：
10.1111/j.1567-1364.2006.00082.x
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发表时间：
2006-06-01
影响因子：
3.2
通讯作者：
Boekhout, Teun
中科院分区：
文献类型：
--
作者：
Bovers, Marjan;Hagen, Ferry;Boekhout, Teun
新型隐球菌和格特隐球菌是引起脑膜脑炎的酵母菌，但其宿主范围和地理分布不同。新型隐球菌在世界范围内发生，主要感染免疫功能低下的患者，而格特隐球菌主要发生在（亚）热带地区，感染健康个体。从患者体内分离出异常新型隐球菌菌株。发现这些菌株是单核、二倍体或非整倍体。扩增片段长度多态性 (AFLP) 和序列分析表明存在 AFLP 基因型 2（新型隐球菌）和 4（格特隐球菌）。这些菌株在血清学上是BD。交配特异性和血清型特异性 PCR 反应表明，这些菌株为 MATa 血清型 D/MAT α 血清型 B。这项研究首次描述了新型隐球菌和格特隐球菌之间天然存在的杂交种。
Cryptococcus neoformans and Cryptococcus gattii are yeasts that cause meningoencephalitis, but that differ in host range and geographical distribution. Cryptococcus neoformans occurs world-wide and mostly infects immunocompromised patients, whereas C. gattii occurs mainly in (sub)tropical regions and infects healthy individuals. Anomalous C. neoformans strains were isolated from patients. These strains were found to be monokaryotic, and diploid or aneuploid. Amplified Fragment Length Polymorphism (AFLP) and sequence analyses indicated that AFLP genotypes 2 (C. neoformans) and 4 (C. gattii) were present. The strains were serologically BD. Mating- and serotype-specific PCR reactions showed that the strains were MATa-serotype D/MAT alpha-serotype B. This study is the first to describe naturally occurring hybrids between C. neoformans and C. gattii.