Characterization of C18-bonded liquid chromatographic stationary phases by Raman spectroscopy: the effect of mobile phase composition.
Characterization of C18-bonded liquid chromatographic stationary phases by Raman spectroscopy: the effect of mobile phase composition.
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通过拉曼光谱表征 C18 键合液相色谱固定相：流动相组成的影响。
DOI：
10.1016/s0021-9673(00)00175-8
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Dorsey,JG
中科院分区：
文献类型：
--
作者：
Doyle,CA;Vickers,TJ;Mann,CK;Dorsey,JG
利用拉曼光谱研究了流动相组成对十八烷基键合硅胶反相液相色谱(RPLC)固定相配体取向的影响。还考察了配基键合密度的影响。目前的实验装置利用一种直接的、非侵入性的柱上方法来研究键合配体在类似于常规RPLC测量中所使用的流速和背压条件下的溶剂依赖构象行为。采用纯净的单组分流动相溶剂，包括水、乙腈、甲醇和氯仿，研究了固定相配体随流动相组成的变化而假想的坍塌和延伸。当流动相组成从有机成分改变为水含量时，没有观察到相崩溃的证据。此外，拉曼光谱测量还可以区分缔合的和游离的乙腈溶剂。
Raman spectroscopy is used to examine the effect of mobile phase composition on the orientation of octadecyl-bonded silica-based reversed-phase liquid chromatographic (RPLC) stationary phase ligands. The effect of ligand bonding density is also investigated. The present experimental set-up utilizes a direct, noninvasive, on-column approach to examine the solvent dependent conformational behavior of the bonded ligands under flow-rate and back pressure conditions similar to those used during conventional RPLC measurements. Neat, single-component, mobile phase solvents including water, acetonitrile, methanol and chloroform are used to investigate the hypothesized collapsing and extension of stationary phase ligands with changes in mobile phase composition. No evidence of phase collapse was observed upon changing the mobile phase composition from an organic to an aqueous content. Also, Raman spectroscopic measurements allowed the differentiation between associated and free acetonitrile solvent.