Sustained concurrent blood pressure and salt/water balance in unrestrained rats.
Sustained concurrent blood pressure and salt/water balance in unrestrained rats.
复制标题
无约束大鼠的持续并发血压和盐/水平衡。
作者：
A. Gill;K. Beyer
详细介绍了在不受约束、不麻醉的自发性高血压大鼠（SHR）中，通过直接测量动脉压，持续同时测量液体体积、电解质摄入和输出的方法。该方法采用全聚四氟乙烯动脉压传导系统、气压输注系统和标准大鼠代谢笼。血压-盐/水平衡研究比较了未手术、假手术和不同输注速率下的SHR和生理盐水正常值，结果表明，以0.5 ml/h的速度输注0.25 N生理盐水可以维持一个专利系统，在3周的时间内高保真地记录收缩压和舒张压，而不会改变体内平衡。基础SHR血压和盐/水平衡的研究表明，SHR主要利用肾脏保水机制在11天内稀释净获得盐。这种持续测量血压的方法似乎代表了在不受约束、不麻醉的大鼠中直接测量血压的显著改进。
Methodology is detailed that provides sustained concurrent measurement of fluid volume and electrolyte intake and output with direct measurement of arterial pressure in unrestrained, unanesthetized spontaneously hypertensive rats (SHR). This method employs an all-Teflon arterial pressure conduction system, an air-pressurized infusion system, and standard rat metabolism cages. Blood pressure-salt/water balance studies comparing SHR unoperated, sham operated, and infused at several infusion rates and saline normalities indicated that infusion of 0.25 N saline at 0.5 ml/h sustained a patent system for high-fidelity recording of systolic and diastolic blood pressure without distortion of homeostasis over a 3-week period. Studies of basal SHR blood pressure and salt/water balance suggested that the SHR primarily used renal water-conserving mechanisms to dilute net acquired salt over an 11-day period. This methodology for sustained blood pressure measurement appears to represent a significant improvement for direct blood pressure measurement in unrestrained, unanesthetized rats.