Basal cells have longest telomeres measured by tissue Q-FISH method in lingual epithelium
Basal cells have longest telomeres measured by tissue Q-FISH method in lingual epithelium
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DOI：
10.1016/j.exger.2008.06.001
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发表时间：
2008-09-01
影响因子：
3.9
通讯作者：
Takubo, Kaiyo
中科院分区：
文献类型：
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作者：
Aida, Junko;Izumiyama-Shimomura, Naotaka;Takubo, Kaiyo
我们使用改进的组织定量荧光原位杂交 (Q-FISH) 方法研究了舌粘膜中单个细胞类型的端粒长度。我们的组织 Q-FISH 方法通过使用端粒：着丝粒比率 (TCR) 来补偿组织切片中部分切割的细胞核。我们使用放置在同一载玻片上的培养细胞块的切片对 TCR 测量值 (NTCR) 进行标准化，从而提高了结果的准确性和可重复性。正常舌粘膜取自 21 名尸检个体。免疫组织化学显示基底细胞中的 p27、p63 和 CK19 呈阳性，副基底细胞中的 Ki-67 呈阳性。 Q-FISH首先显示，NTCR在基底细胞中显着最高，在棘细胞中最低，而且每种细胞类型的端粒长度都以一定的速率退化。三种上皮细胞类型之间的 NTCR 显着相关性得到证实。目前的发现似乎支持干细胞存在于舌上皮基底层的理论。各细胞层中端粒长度随着年龄的增长而减少，与口腔粘膜细胞增殖和分化的端粒生物学理论一致。 (C) 2008 Elsevier Inc. 保留所有权利。
We investigated the telomere lengths of individual cell types in lingual mucosa using an improved tissue quantitative fluorescence in situ hybridization (Q-FISH) method. Our tissue Q-FISH method compensates for partially cut nuclei in a tissue section by using the telomere:centromere ratio (TCR). We normalized our TCR measurements (NTCR) using a section from a block of cultured cells placed on the same slide, thus improving the accuracy and reproducibility of the results. Normal lingual mucosa was obtained from 21 autopsied individuals. Immunohistochemistry showed positivity mainly for p27, p63, and CK19 in basal cells, and for Ki-67 in parabasal cells. Q-FISH revealed that NTCR was significantly highest in basal cells and lowest in prickle cells, and also that telomere length regressed at a certain rate in each cell type, firstly. Significant correlations of NTCR among the three epithelia] cell types were demonstrated. The present findings appear to support the theory that stem cells exist in the basal layer of the lingual epithelium. The reduction of telomere length with age and in each cell layer is consistent with the telomere biology theory of cell proliferation and differentiation in oral mucosa. (C) 2008 Elsevier Inc. All rights reserved.