Irrigation management and phosphorus addition alter the abundance of carbon dioxide-fixing autotrophs in phosphorus-limited paddy soil
Irrigation management and phosphorus addition alter the abundance of carbon dioxide-fixing autotrophs in phosphorus-limited paddy soil
复制标题
灌溉管理和磷添加改变了磷限制性水稻土中二氧化碳固定自养生物的丰度
DOI：
10.1093/femsec/fix154
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发表时间：
2017
影响因子：
4.2
通讯作者：
Wu Jinshui
中科院分区：
文献类型：
--
作者：
Wu Xiaohong;Ge Tida;Yan Wende;Zhou Juan;Wei Xiaomeng;Chen Liang;Chen Xiangbi;Nannipieri Paolo;Wu Jinshui
研究了磷和灌溉方式对固氮自养生物标记基因（cbbL、cbbM、accA和aclB）丰度的交互作用。本研究以磷素限制的水稻土为试验材料，采用干湿交替（AWD）和连续淹水（CF）两种灌溉方式，进行了水稻微宇宙试验。测定了根际土壤（RS）和非根际土壤（BS）中细菌16 S rRNA、古细菌16 S rRNA、cbbL、cbbM、accA和aclB基因的丰度。施磷显著改变了CF处理下BS土壤的性质，促进了细菌、固氮菌和CO2固定基因的丰度，但对AWD处理下BS土壤细菌和CO2固定自养菌标记基因的丰度产生了负面影响。固定CO2的自养生物对磷肥的反应取决于灌溉管理方法。冗余度分析表明，54%的功能标记基因丰度的变异可以由灌溉方式、磷肥和Olsen-P含量解释，而根际效应没有显著影响。与AWD处理相比，CF下施磷肥更有利于提高土壤固碳自养菌的丰度，是一种理想的提高土壤固碳的灌溉管理方法。
In this study, we assessed the interactive effects of phosphorus (P) application and irrigation methods on the abundances of marker genes (cbbL,cbbM,accAandaclB) of CO2-fixing autotrophs. We conducted rice-microcosm experiments using a P-limited paddy soil, with and without the addition of P fertiliser (P-treated-pot (P) versus control pot (CK)), and using two irrigation methods, namely alternate wetting and drying (AWD) and continuous flooding (CF). The abundances of bacterial 16S rRNA, archaeal 16S rRNA,cbbL,cbbM,accAandaclBgenes in the rhizosphere soil (RS) and bulk soil (BS) were quantified. The application of P significantly altered the soil properties and stimulated the abundances of Bacteria, Archaea and CO2-fixation genes under CF treatment, but negatively influenced the abundances of Bacteria and marker genes of CO2-fixing autotrophs in BS soils under AWD treatment. The response of CO2-fixing autotrophs to P fertiliser depended on the irrigation management method. The redundancy analysis revealed that 54% of the variation in the functional marker gene abundances could be explained by the irrigation method, P fertiliser and the Olsen-P content; however, the rhizosphere effect did not have any significant influence. P fertiliser application under CF was more beneficial in improving the abundance of CO2-fixing autotrophs compared to the AWD treatment; thus, it is an ideal irrigation management method to increase soil carbon fixation.