Effects of hyperfibrinogenemia on vasculature of C57BL/6 mice with and without atherogenic diet
Effects of hyperfibrinogenemia on vasculature of C57BL/6 mice with and without atherogenic diet
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DOI：
10.1161/01.atv.0000041037.06509.c2
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发表时间：
2003-01-01
影响因子：
8.7
通讯作者：
Lord, ST
中科院分区：
文献类型：
--
作者：
Gulledge, AA;McShea, C;Lord, ST
目的：纤维蛋白原升高与严重的动脉粥样硬化有关，其定义为缺血事件的发生，但这种相关性的机制基础尚不清楚。为了研究这种关系，我们研究了患有高纤维蛋白原血症(纤维蛋白原升高)的转基因小鼠的自发动脉粥样硬化和饮食诱导的动脉粥样硬化。方法和结果-正常和转基因小鼠被喂以致动脉粥样硬化的饮食或简单的种鸡饲料。我们测量了血浆纤维蛋白原水平，发现纤维蛋白原随年龄和饮食的增加而增加。8至12个月后，制备主动脉切片并染色，计数、测量含脂病变并评估其成熟度--饲养员喂养的一小部分小鼠自发出现充满脂质的沉积物；几乎所有喂食饲料的小鼠都出现典型的脂肪条纹型病变。形态计量学分析表明，无论是数量还是数量。病变的大小受纤维蛋白原水平或基因型别的影响。结论：我们的数据显示，纤维蛋白原浓度和基因型别对动脉粥样硬化病变的起始、初始发展或早期进展均无统计学意义的影响。
Objective-Elevated fibrinogen is correlated with severe atherosclerosis, as defined by the occurrence of ischemic events, but the mechanistic basis for this correlation remains unknown. To study this relationship, we examined spontaneous and diet-induced atherosclerosis in transgenic mice with hyperfibrinogenemia (elevated fibrinogen).Methods and Results-Normal and transgenic mice were fed either an atherogenic diet or simple breeder chow. We measured plasma fibrinogen levels and identified an age-dependent and diet-dependent increase in fibrinogen. After 8 to 12 months, aortic sections were prepared and stained, and lipid-containing lesions were counted, measured, and assessed for maturity-Lipid-filled deposits appeared spontaneously in a small number of mice on breeder chow; typical fatty streak-type lesions appeared in almost all of the diet-fed animals. Morphometric analysis showed that neither the number nor the. size of lesions was influenced by either fibrinogen level or genotype.Conclusions-Our data showed that neither fibrinogen concentration nor genotype had a statistically significant effect on the initiation, initial growth, or early progression of atherosclerotic lesions.