Antiviral activity of angelicin against gammaherpesviruses
Antiviral activity of angelicin against gammaherpesviruses
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DOI：
10.1016/j.antiviral.2013.07.009
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发表时间：
2013-10-01
影响因子：
7.6
通讯作者：
Song, Moon Jung
中科院分区：
文献类型：
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作者：
Cho, Hye-Jeong;Jeong, Seon-Gyeong;Song, Moon Jung
人类伽玛疱疹病毒包括爱泼斯坦-巴尔病毒(Epstein-Barr Virus，EBV)和卡波西肉瘤相关疱疹病毒(Kaposi‘s肉瘤相关疱疹病毒，KSHV)，它们在宿主体内存活并引起各种恶性肿瘤，是重要的病原体。然而，几乎没有抗病毒药物可以有效地控制伽马疱疹病毒的复制。在这里，我们鉴定了当归对小鼠伽马疱疹病毒68(MHV-68)的抗病毒活性，该病毒在基因和生物学上与人类伽马疱疹病毒相关。当归是一种呋喃香豆素类天然存在的三环芳香化合物，在病毒进入后以剂量依赖的方式有效地抑制MHV-68的裂解复制。当归素抗病毒作用的IC50为28.95 mU/M，而当归素抗病毒的CC50>2600 mU/M。此外，当归素能有效地抑制12-O-十四酰佛波醇-13-乙酸酯(TPA)诱导的人伽马超前病毒在EBV和KSHV感染细胞中的裂解复制。综上所述，这些结果表明，MHV-68可以作为一种有用的工具来筛选新型抗病毒药物来对抗人类伽马疱疹病毒，而当归素有可能为开发抗病毒药物提供一个先导结构。(C)2013爱思唯尔B.V.保留所有权利。
Human gammaherpesviruses including Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) are important pathogens as they persist in the host and cause various malignancies. However, few antiviral drugs are available to efficiently control gammaherpesvirus replication. Here we identified the antiviral activity of angelicin against murine gammaherpesvirus 68 (MHV-68), genetically and biologically related to human gammaherpesviruses. Angelicin, a furocoumarin naturally occurring tricyclic aromatic compound, efficiently inhibited lytic replication of MHV-68 in a dose-dependent manner following the virus entry. The IC50 of angelicin antiviral activity was estimated to be 28.95 mu M, while the CC50 of angelicin was higher than 2600 mu M. Furthermore, incubation with angelicin efficiently inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced lytic replication of human gammaherpresviruses in both EBV- and KSHV-infected cells. Taken together, these results suggest that MHV-68 can be a useful tool to screen novel antiviral agents against human gammaherepsviruses and that angelicin may provide a lead structure for the development of antiviral drug against gammaherpesviruses. (C) 2013 Elsevier B.V. All rights reserved.