Phosphorylation of Thr-225 and Ser-262 on ERD7 Promotes Age-Dependent and Stress-Induced Leaf Senescence through the Regulation of Hydrogen Peroxide Accumulation in Arabidopsis thaliana.
Phosphorylation of Thr-225 and Ser-262 on ERD7 Promotes Age-Dependent and Stress-Induced Leaf Senescence through the Regulation of Hydrogen Peroxide Accumulation in Arabidopsis thaliana.
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DOI：
10.3390/ijms25021328
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发表时间：
2024-01-22
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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As the final stage of leaf development, leaf senescence is affected by a variety of internal and external signals including age and environmental stresses. Although significant progress has been made in elucidating the mechanisms of age-dependent leaf senescence, it is not clear how stress conditions induce a similar process. Here, we report the roles of a stress-responsive and senescence-induced gene, ERD7 (EARLY RESPONSIVE TO DEHYDRATION 7), in regulating both age-dependent and stress-induced leaf senescence in Arabidopsis. The results showed that the leaves of erd7 mutant exhibited a significant delay in both age-dependent and stress-induced senescence, while transgenic plants overexpressing the gene exhibited an obvious accelerated leaf senescence. Furthermore, based on the results of LC-MS/MS and PRM quantitative analyses, we selected two phosphorylation sites, Thr-225 and Ser-262, which have a higher abundance during senescence, and demonstrated that they play a key role in the function of ERD7 in regulating senescence. Transgenic plants overexpressing the phospho-mimetic mutant of the activation segment residues ERD7T225D and ERD7T262D exhibited a significantly early senescence, while the inactivation segment ERD7T225A and ERD7T262A displayed a delayed senescence. Moreover, we found that ERD7 regulates ROS accumulation by enhancing the expression of AtrbohD and AtrbohF, which is dependent on the critical residues, i.e., Thr-225 and Ser-262. Our findings suggest that ERD7 is a positive regulator of senescence, which might function as a crosstalk hub between age-dependent and stress-induced leaf senescence.
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影响因子：
16
作者：
Kadota, Yasuhiro;Sklenar, Jan;Zipfel, Cyril
通讯作者：
Zipfel, Cyril
影响因子：
4.9
作者：
Barajas-Lopez, Juan de Dios;Tiwari, Arjun;Fujii, Hiroaki
通讯作者：
Fujii, Hiroaki
影响因子：
7.4
作者：
Gan, SS;Amasino, RM
通讯作者：
Amasino, RM
影响因子：
5.6
作者：
Gallino JP;Ruibal C;Casaretto E;Fleitas AL;Bonnecarrère V;Borsani O;Vidal S
通讯作者：
Vidal S
影响因子：
3.7
作者：
Joshi DC;Bakowska JC
通讯作者：
Bakowska JC