GONOCOCCAL STRAINS FROM HOMOSEXUAL MEN HAVE OUTER MEMBRANES WITH REDUCED PERMEABILITY TO HYDROPHOBIC MOLECULES
GONOCOCCAL STRAINS FROM HOMOSEXUAL MEN HAVE OUTER MEMBRANES WITH REDUCED PERMEABILITY TO HYDROPHOBIC MOLECULES
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DOI：
10.1128/iai.37.2.432-438.1982
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发表时间：
1982-01-01
影响因子：
3.1
通讯作者：
HOLMES, KK
中科院分区：
文献类型：
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作者：
MORSE, SA;LYSKO, PG;HOLMES, KK
命名为佩纳、penB和mtr的基因座对淋病奈瑟菌的青霉素G耐药性有相加作用; mtr基因座还赋予对疏水染料、洗涤剂和抗生素的耐药性。Env突变抑制mtr和penB的表型表达，并导致对各种疏水分子的敏感性增加。显然，宿主环境在选择具有这些特定外膜表型的淋球菌菌株中是重要的。因此，mtr菌株应该在疏水分子含量高的环境中占优势。为了验证这一假设，对152株N.确定淋病。在这项研究中使用的直肠和尿道分离株从58名同性恋男性，尿道分离株从55名异性恋男性，和宫颈和直肠分离株从39名异性恋女性。从43名同性恋男子的菌株与异性恋男子的生长型和隔离的年份和季节相匹配。细胞被膜表型的基础上，确定其对各种疏水化合物的耐药性的每一个菌株。通过遗传转化证实了mtr菌株的身份。在配对中，MTR菌株在来自同性恋男性的分离株中的流行率显著高于来自异性恋男性的分离株（P = 0.03）。通过凝固酶进行血清分型，19株mtr菌株中有17株与131株非mtr菌株中的76株属于凝固酶组WII（P = 0.01）。共凝集组WII菌株也与同性恋相关（P = 0.02）。还测试了淋球菌对粪便脂质的抗性。MTR菌株比非MTR菌株对粪便脂质的生长抑制更具抗性。
Loci designated penA, penB and mtr contribute additively to penicillin G resistance in Neisseria gonorrhoeae; the mtr locus also confers resistance to hydrophobic dyes, detergents and antibiotics. Env mutations suppress the phenotypic expression of mtr and penB and are responsible for increased sensitivity to various hydrophobic molecules. Evidently, the host environment is important in the selection of gonococcal strains with these particular outer membrane phenotypes. Thus, mtr strains should predominate in environments that are high in hydrophobic molecules. To test this hypothesis, the outer membrane phenotype of 152 strains of N. gonorrhoeae was determined. Rectal and urethral isolates from 58 homosexual men, urethral isolates from 55 heterosexual men, and cervical and rectal isolates from 39 heterosexual women were used in this study. Strains from 43 of the homosexual men were matched with those from heterosexual men with respect to auxotype and year and season of isolation. Cell envelope phenotype was determined for each strain on the basis of its resistance to various hydrophobic compounds. The identity of mtr strains was confirmed by genetic transformation. Among the matched pairs, mtr strains were significantly more prevalent among isolates from homosexual men than among those from heterosexual men (P = 0.03). Serogrouping by coagglutination demonstrated that 17 of 19 mtr strains vs. 76 of 131 non-mtr strains belonged to coagglutination group WII (P = 0.01). Coagglutination group WII strains were also associated with homosexuality (P = 0.02). Gonococci were also tested for resistance to fecal lipids. Mtr strains were more resistant to growth inhibition by fecal lipids than were non-mtr strains.