Genome-wide identification and expression analysis of heat shock protein gene family in cassava
Genome-wide identification and expression analysis of heat shock protein gene family in cassava
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DOI：
10.1002/tpg2.20407
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发表时间：
2023-10-29
期刊：
影响因子：
4.2
通讯作者：
Min，Yi
中科院分区：
文献类型：
--
作者：
Wang，Changyi;Ran，Fangfang;Min，Yi
热休克蛋白是重要的分子伴侣，参与植物的生长和逆境反应。然而，热休克蛋白家族的成员在木薯中的研究一直很少。在本研究中，在木薯基因组中发现了225MeHspgene，其遗传结构在每个亚家族中都表现出相对保守的特征。225MeHsp1基因呈随机染色体分布，至少有74对节段性重复的MeHsp1基因。共鉴定出11个重复的MeHsp2基因。顺式元件分析揭示了MeHsps在植物适应环境中的重要性。对蛋白质相互作用的预测表明，MeHsp70-20可能在相互作用网络中发挥关键的调节作用。此外，利用批量转录和单细胞转录数据分析了MeHsps在不同组织和细胞亚群中的表达谱。一些亚家族基因在转录组中表现出独特的表达模式，并被选择用于单细胞转录组的详细分析。定量实时聚合酶链式反应(qRT-PCR)揭示了这些基因在温度胁迫下的表达模式，进一步支持了顺式作用元件的预测。本研究为了解MeHsps基因的功能特征以及MeHsps之间的进化关系提供了有价值的信息。
Heat shock proteins are important molecular chaperones that are involved in plant growth and stress responses. However, members of theHspfamily have been poorly studied in cassava. In this study, 225MeHspgenes were identified in the cassava genome, and their genetic structures exhibited relatively conserved features within each subfamily. The 225MeHspgenes showed random chromosomal distribution, and at least 74 pairs of segmentally duplicatedMeHspgenes. Eleven tandemly duplicatedMeHspgenes were identified. Cis‐element analysis revealed the importance ofMeHsps in plant adaptations to the environment. The prediction of protein interactions suggested thatMeHsp70‐20 may play a critical regulatory role in the interactive network. Furthermore, the expression profiles ofMeHsps in different tissues and cell subsets were analyzed using bulk transcriptomics and single‐cell transcriptomic data. Several subfamily genes exhibited unique expression patterns in the transcriptome and were selected for detailed analysis of the single‐cell transcriptome. Quantitative real‐time polymerase chain reaction (qRT‐PCR) revealed the expression patterns of these genes under temperature stress, further supporting the prediction of cis‐acting elements. This study provides valuable information for understanding the functional characteristics ofMeHspgenes and the evolutionary relationships betweenMeHsps.