Molecular taxonomy of major neuronal classes in the adult mouse forebrain
Molecular taxonomy of major neuronal classes in the adult mouse forebrain
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DOI：
10.1038/nn1618
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发表时间：
2006-01-01
影响因子：
25
通讯作者：
Nelson, SB
中科院分区：
文献类型：
--
作者：
Sugino, K;Hempel, CM;Nelson, SB
识别构成哺乳动物前脑的神经元细胞类型是神经科学中尚未解决的核心问题。全球基因表达谱提供了一个潜在的公正的方式来评估神经元之间的功能关系。在这里，我们对成年小鼠前脑中的12个神经元群体进行了微阵列分析。其中5个群体选自扣带皮层，包括GABA能中间神经元和锥体神经元的几种亚型。其余7个来自躯体感觉皮层、海马、杏仁核和丘脑。使用这些表达谱，我们能够构建一个分类树，反映这些群体之间的预期主要关系，如皮质中间神经元和投射神经元之间的区别。分类树表明，即使在一个单一的区域高度异质性的基因表达。该数据集对于未知神经元亚型的分类、特异性表达基因的研究和特定神经元回路元件的遗传操作是有用的。
Identifying the neuronal cell types that comprise the mammalian forebrain is a central unsolved problem in neuroscience. Global gene expression profiles offer a potentially unbiased way to assess functional relationships between neurons. Here, we carried out microarray analysis of 12 populations of neurons in the adult mouse forebrain. Five of these populations were chosen from cingulate cortex and included several subtypes of GABAergic interneurons and pyramidal neurons. The remaining seven were derived from the somatosensory cortex, hippocampus, amygdala and thalamus. Using these expression profiles, we were able to construct a taxonomic tree that reflected the expected major relationships between these populations, such as the distinction between cortical interneurons and projection neurons. The taxonomic tree indicated highly heterogeneous gene expression even within a single region. This dataset should be useful for the classification of unknown neuronal subtypes, the investigation of specifically expressed genes and the genetic manipulation of specific neuronal circuit elements.