Estimation of carbon biomass and community structure of planktonic bacteria in Lake Biwa using respiratory quinone analysis
Estimation of carbon biomass and community structure of planktonic bacteria in Lake Biwa using respiratory quinone analysis
复制标题
利用呼吸醌分析估算琵琶湖浮游细菌的碳生物量和群落结构
DOI：
10.1007/s10201-013-0402-3
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发表时间：
2013
期刊：
影响因子：
1.6
通讯作者：
S.
中科院分区：
文献类型：
--
作者：
Takasu;H.;Kunihiro;T.;Nakano;S.
细菌呼吸醌（RQ）的浓度和生物量之间的关系进行了评估的琵琶湖细菌组合，以评估细菌RQ浓度作为细菌碳的指示剂的效用。由RQ浓度估计的生物量与细胞体积估计的生物量相关性良好，表明RQ浓度是细菌生物量的适当指标。每单位RQ（碳转换因子）细菌的碳含量估计为0.67 mg C nmol RQ−1。细菌碳生物量，这是估计从RQ浓度使用转换因子，范围在0.008和0.054毫克C L−1（平均0.025毫克C L−1）在5米深处和0.010和0.024毫克C L−1（平均0.015毫克C L−1）在70米深处。泛醌-8的细菌占优势的上层和下层。与传统的图像分析相比，细菌RQ分析是一种同时确定细菌生物量和群落的不太费力的方法。
The relationship between bacterial respiratory quinone (RQ) concentration and biomass was assessed for Lake Biwa bacterial assemblages to evaluate the utility of bacterial RQ concentration as an indicator of bacterial carbon. The biomass estimated from the RQ concentration correlated well with that from cell volume, indicating that RQ concentration is an appropriate indicator of bacterial biomass. The estimated carbon content per unit of RQ (carbon conversion factor) of bacteria was 0.67 mg C nmol RQ−1. Bacterial carbon biomass, which was estimated from the RQ concentration using the conversion factor, ranged between 0.008 and 0.054 mg C L−1(average 0.025 mg C L−1) at 5 m depth and between 0.010 and 0.024 mg C L−1(average 0.015 mg C L−1) at 70 m depth. Ubiquinone-8-containing bacteria dominated the epilimnion and hypolimnion. Compared to conventional image analysis, bacterial RQ analysis is a less laborious method of simultaneously determining bacterial biomass and community.