Masseter inflammation differentially regulates three nitric oxide synthases in the rat trigeminal subnucleus caudalis.
Masseter inflammation differentially regulates three nitric oxide synthases in the rat trigeminal subnucleus caudalis.
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DOI：
10.1016/j.archoralbio.2012.03.001
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发表时间：
2012-08
影响因子：
3
通讯作者：
Ro JY
中科院分区：
文献类型：
--
作者：
Chun YH;Auh QS;Lee J;Ro JY
本研究的目的是评估实验性肌炎条件下三叉神经感觉核复合体（Vc）尾亚核中三种一氧化氮合酶（NOS）的表达水平变化，即诱导型一氧化氮合酶（iNOS）、神经元一氧化氮合酶（nNOS）和内皮一氧化氮合酶（eNOS）。雄性斯普拉道利大鼠的咬肌中注射了炎症剂、完全弗氏佐剂（CFA）或辣椒素。在立即（30和60分钟）和更长（1、3、7天）时间点提取含有Vc的脑干区域，通过蛋白质印迹技术检查三种NOS蛋白水平的变化。随后进行RT-PCR实验来验证iNOS mRNA的变化。注射 CFA 后，三种 NOS 蛋白的水平在即时时间点没有显着变化。然而，CFA 诱导炎症后 3 天，iNOS mRNA 和蛋白质显着上调。在任何较长时间点，nNOS 和 eNOS 均未显示出蛋白质水平的显着变化。我们最近报告称，咬肌注射辣椒素会在即时时间点上调所有三种 NOS，但在较长时间点并未导致显着变化。急性和慢性肌肉炎症差异性地调节 Vc 中三种 NOS 的表达。这些数据表明，炎症条件下每种 NOS 在颅面肌肉疼痛处理中的贡献可以通过不同的时间曲线来预测。
The aim of the present study was to evaluate changes in expression levels of three nitric oxide synthases (NOSs), namely inducible NOS (iNOS), neuronal NOS (nNOS) and endothelial NOS (eNOS), in the subnucleus caudalis of the trigeminal sensory nuclear complex (Vc) under experimental myositis conditions. Male Sprague Dawley rats were injected with an inflammatory agent, complete Freund’s adjuvant (CFA), or capsaicin in the masseter muscle. The brainstem region containing the Vc was extracted at both immediate (30 and 60 min) and longer (1, 3, 7 days) time points to examine the changes in the three NOS protein levels via the Western blot technique. Subsequently, the RT-PCR experiments were carried out to verify the changes in iNOS mRNA. Following the injections of CFA, there were no significant changes in the level of the three NOS proteins at the immediate time points. However, there was a significant upregulation of iNOS mRNA and protein 3 days after CFA-induced inflammation. Neither nNOS nor eNOS showed significant changes in the protein level at any of the longer time points. Capsaicin injection in the masseter, which we recently reported to upregulate all three NOS at the immediate time points, did not result in significant changes at longer time points. Acute and chronic muscle inflammation differentially modulates the expression of the three NOS in the Vc. These data suggest that the contribution of each NOS in craniofacial muscle pain processing under inflammatory conditions may be anticipated with distinct temporal profiles.
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