PLASMID DNA ENTRY INTO POSTMITOTIC NUCLEI OF PRIMARY RAT MYOTUBES
PLASMID DNA ENTRY INTO POSTMITOTIC NUCLEI OF PRIMARY RAT MYOTUBES
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DOI：
10.1073/pnas.92.10.4572
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发表时间：
1995-05-09
影响因子：
11.1
通讯作者：
WOLFF, JA
中科院分区：
文献类型：
--
作者：
DOWTY, ME;WILLIAMS, P;WOLFF, JA
这些研究旨在阐明哺乳动物细胞中DNA核转运的机制。将生物素或金标记的质粒和大肠杆菌β-半乳糖苷酶或萤火虫荧光素酶的质粒DNA表达载体显微注射到培养的原代大鼠肌管的细胞质中。质粒DNA在高达70%的注射肌管中表达，这表明它进入了完整的有丝分裂后的细胞核。质粒DNA的核转运通过核孔发生，其过程与其他大的嗜核大分子相同。大部分注射的质粒DNA被细胞质元件隔离。对质粒DNA核转运的理解为提高基因转移的效率提供了基础。
These studies were initiated to elucidate the mechanism of DNA nuclear transport in mammalian cells. Biotin- or gold-labeled plasmid and plasmid DNA expression vectors for Escherichia coli beta-galactosidase or firefly luciferase were microinjected into the cytoplasm of primary rat myotubes in culture. Plasmid DNA was expressed in up to 70% of the injected myotubes, which indicates that it entered intact, postmitotic nuclei. The nuclear transport of plasmid DNA occurred through the nuclear pore by a process common to other large karyophilic macromolecules. The majority of the injected plasmid DNA was sequestered by cytoplasmic elements. This understanding of plasmid DNA nuclear transport provides a basis for increasing the efficiency of gene transfer.