High human T-cell lymphotropic virus type I proviral DNA load with polyclonal integration in peripheral blood mononuclear cells of French West Indian, Guianese, and African patients with tropical spastic paraparesis.
High human T-cell lymphotropic virus type I proviral DNA load with polyclonal integration in peripheral blood mononuclear cells of French West Indian, Guianese, and African patients with tropical spastic paraparesis.
复制标题
法属西印度群岛、圭亚那和非洲热带痉挛性截瘫患者外周血单核细胞中人类 T 细胞嗜淋巴细胞病毒 I 型前病毒 DNA 载量高且多克隆整合。
作者：
A. Gessain;F. Saal;O. Gout;M. Daniel;G. Flandrin;G. de Thé;J. Peries;F. Sigaux
采用Southern blot方法检测了热带痉性截瘫（TSP）患者外周血单个核细胞（PBMC） DNA中人嗜t细胞病毒I型（HTLV-I）前病毒整合状态和HTLV-I感染的血清学证据。还研究了PBMC的表面表型和形态学。在研究的10例患者的PBMC中发现了多克隆HTLV-I前病毒整合，无论其地理来源（法属西印度群岛、法属圭亚那和非洲）、临床病程或HTLV-I抗体滴度如何。此外，通过稀释实验和假设一个细胞中只存在一个HTLV-I前病毒DNA拷贝，我们估计HTLV-I整合存在于3%至15%的PBMC中。所有10例TSP/HTLV-I患者平均有10%的淋巴细胞异常，表现为细胞核畸形或成人t细胞白血病/淋巴瘤（ATL）样特征。此外，在大多数患者中观察到CD4/CD8比值升高与高水平DR表达的活化T细胞的存在相关。我们在TSP/HTLV-I患者中观察到的病毒阳性PBMC的显著频率和HTLV-I前病毒DNA的重要负荷可能在这种最近发现的临床病毒学实体的发病机制中起重要作用。
Human T-cell lymphotropic virus type I (HTLV-I) proviral integration status was examined by Southern blot analysis in peripheral blood mononuclear cell (PBMC) DNA from patients presenting a tropical spastic paraparesis (TSP) and serological evidence of HTLV-I infection. Surface phenotype and morphological aspects of PBMC were also studied. A polyclonal HTLV-I proviral integration was found in the PBMC of the 10 patients studied irrespective of their geographical origin (French West Indies, French Guiana, and Africa), the duration of their clinical illness, or the HTLV-I antibody titer. Furthermore, by dilution experiments and hypothesizing that only one copy of HTLV-I proviral DNA is present in one cell, we estimated that this HTLV-I integration is present in 3% to 15% of their PBMC. All 10 TSP/HTLV-I patients studied had an average of 10% of their lymphocytes abnormal, presenting either a misshapen nucleus or an adult T-cell leukemia/lymphoma (ATL)-like feature. Moreover, an elevated CD4/CD8 ratio associated with the presence of activated T cells with a high level of DR expression was observed in most patients. The significant frequency of viral-positive PBMC and the important load of HTLV-I proviral DNA that we observed in TSP/HTLV-I patients might play an important role in the pathogenesis of this recently identified clinico-virological entity.