Size-selective separation of DNA fragments by using lysine-functionalized silica particles.
Size-selective separation of DNA fragments by using lysine-functionalized silica particles.
复制标题
使用赖氨酸功能化二氧化硅颗粒对 DNA 片段进行尺寸选择性分离
DOI：
10.1038/srep22029
复制
发表时间：
2016-02-25
影响因子：
4.6
通讯作者：
Yang W
中科院分区：
文献类型：
--
作者：
Liu L;Guo Z;Huang Z;Zhuang J;Yang W
In this work, a facile and efficient approach has been demonstrated for size-selective separation of DNA fragments by using lysine-functionalized silica particles. At a given pH, the environmental ionic strength can be utilized to alter the electrostatic interactions of lysine-functionalized silica particles with DNA fragments and in turn the DNA fragments on the silica particle surfaces, which exhibits a clear dependence on the DNA fragment sizes. By carefully adjusting the environmental pH and salt concentration, therefore, the use of the lysine-functionalized silica particles allows effective separation of binary and ternary DNA mixtures, for example, two different DNA fragments with sizes of 101 and 1073 bp, 101 and 745 bp, 101 and 408 bp, respectively, and three different DNA fragments with sizes of 101, 408 and 1073 bp.
