An Analytical-Experimental Approach to Quantifying the Effects of Static Magnetic Fields for Cell Culture Applications
An Analytical-Experimental Approach to Quantifying the Effects of Static Magnetic Fields for Cell Culture Applications
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DOI：
10.3390/app10020531
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发表时间：
2020-01-01
影响因子：
2.7
通讯作者：
Zapata, Manuel
中科院分区：
文献类型：
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作者：
Ferrada, Pablo;Rodriguez, Sebastian;Zapata, Manuel
本工作旨在研究静磁场（SMF）对细胞培养的影响。将玻璃烧瓶填充有液体介质，其被永磁体包围。通过管引入空气以注入气泡。两个磁铁配置，北和南，被用来作为扰动。生长在培养基1和2中的栅藻和加的斯微绿球藻经受泡状流和SMF。培养基之间的差异主要是由于电导率（培养基1为0.09 S/m，培养基2为4.3 S/m）。焦耳耗散（P）随着磁通量密度（B-0）而增加，在介质2中比在1中高4个数量级。相反，时间常数（tau（P））取决于B-0，对于培养基1几乎是恒定的，而对于培养基2则在449 s/T时减小。在B-0 = 0.5 T时，介质1和介质2中相同的tau（P）（235 s）发生耗散。在种属1中，SMF效应具有抑制作用。对于种属2，观察到较高的酶活性。对于超氧化物歧化酶，与对照值相比，北方配置的相对差异为78%，南方配置的相对差异为115%。对于过氧化氢酶，与对照条件相比，北配置和南配置的差异分别为29%和23%。
This work aimed to study the effects of static magnetic fields (SMFs) on cell cultures. A glass flask was filled with a liquid medium, which was surrounded by permanent magnets. Air was introduced through a tube to inject bubbles. Two magnet configurations, north and south, were used as perturbation. Scenedesmus obliquus and Nannochloropsis gaditana, growing in Medium 1 and 2, were subjected to the bubbly flow and SMFs. Differences between media were mainly due to conductivity (0.09 S/m for Medium 1 and 4.3 S/m for Medium 2). Joule dissipation (P) increased with the magnetic flux density (B-0), being 4 orders of magnitude higher in Medium 2 than in 1. Conversely, the time constant (tau(P)) depended on B-0, being nearly constant for Medium 1 and decreasing at 449 s/T for Medium 2. Dissipation occurred with the same tau(P) (235 s) in Medium 1 and 2 at B-0 = 0.5 T. In Species 1, the SMF effect was inhibitory. For Species 2, a higher enzymatic activity was observed. For superoxide dismutase, the relative difference was 78% with the north and 115% with the south configuration compared to the control values. For the catalase, differences of 29% with the north and 23% with the south configuration compared to control condition were obtained.