An evolutionary view of functional diversity in family 1 glycosyltransferases
An evolutionary view of functional diversity in family 1 glycosyltransferases
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DOI：
10.1111/j.1365-313x.2011.04493.x
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发表时间：
2011-04-01
期刊：
影响因子：
7.2
通讯作者：
Hanada, Kousuke
中科院分区：
文献类型：
--
作者：
Yonekura-Sakakibara, Keiko;Hanada, Kousuke
糖基转移酶（GT）（EC 2.4.x.y）催化糖部分转移到广泛的受体分子，如糖、脂质、蛋白质、核酸、抗生素和其他小分子，包括植物次生代谢物。这些酶可分为至少92个家族，其中家族1糖基转移酶（GT 1），通常称为UDP糖基转移酶（UGT），是植物界中最大的。为了了解UGT在陆地植物进化过程中如何在数量和功能上扩展，我们筛选了六种植物（小立碗藓、卷柏、胡杨、水稻、拟南芥和琴状拟南芥）的基因组序列，以确定是否存在保守的UGT蛋白结构域。UGT基因的系统发育分析显示，UGT的显着扩张，与谱系特异性和较高的重复率在维管植物后，分歧立碗藓。这6个物种的UGT分为24个直链组，其中包含来自这6个物种共同祖先的基因。一些orthopathic组包含多个UGT家族与已知的功能，这表明UGT歧视化合物作为基板在一个谱系特异性的方式。只有一个UGT家族的直翅目类群往往在植物中起着至关重要的作用，这表明这种UGT家族可能因为进化的限制而没有扩大。
P>Glycosyltransferases (GTs) (EC 2.4.x.y) catalyze the transfer of sugar moieties to a wide range of acceptor molecules, such as sugars, lipids, proteins, nucleic acids, antibiotics and other small molecules, including plant secondary metabolites. These enzymes can be classified into at least 92 families, of which family 1 glycosyltransferases (GT1), often referred to as UDP glycosyltransferases (UGTs), is the largest in the plant kingdom. To understand how UGTs expanded in both number and function during evolution of land plants, we screened genome sequences from six plants (Physcomitrella patens, Selaginella moellendorffii, Populus trichocarpa, Oryza sativa, Arabidopsis thaliana and Arabidopsis lyrata) for the presence of a conserved UGT protein domain. Phylogenetic analyses of the UGT genes revealed a significant expansion of UGTs, with lineage specificity and a higher duplication rate in vascular plants after the divergence of Physcomitrella. The UGTs from the six species fell into 24 orthologous groups that contained genes derived from the common ancestor of these six species. Some orthologous groups contained multiple UGT families with known functions, suggesting that UGTs discriminate compounds as substrates in a lineage-specific manner. Orthologous groups containing only a single UGT family tend to play a crucial role in plants, suggesting that such UGT families may have not expanded because of evolutionary constraints.