SEPARATIONS OF CHEMICALLY DIFFERENT PARTICLES BY CAPILLARY ELECTROPHORESIS
SEPARATIONS OF CHEMICALLY DIFFERENT PARTICLES BY CAPILLARY ELECTROPHORESIS
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DOI：
10.1021/ac00221a014
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发表时间：
1990-11-15
影响因子：
7.4
通讯作者：
BALLOU, NE
中科院分区：
文献类型：
--
作者：
JONES, HK;BALLOU, NE
毛细管电泳已经成功地实现了化学性质不同的乳胶颗粒（不同数量的羧酸盐或硫酸盐基团）混合物的快速、良好分离。确定了柱内径和柱长、缓冲液浓度和pH、外加电压等分离的最佳实验条件。测定电泳迁移次数和迁移率。分离的特点是理论板的数量，选择性和分辨率在不同的pH值和施加的电压值。理论板的数量从400到3000不等。选择性范围约为0.1至0.4，分辨率范围为0.8至2.3。电泳迁移率变化，特别是在较高的施加电压下。
Fast, well-resolved separations of mixtures of chemically different latex particles (different numbers of attached carboxylate or sulfate groups) have been successfully achieved by capillary electrophoresis. Best experimental conditions of column inside diameter and length, buffer concentration and pH, and applied voltage were identified for the separations. Electrophoretic migration times and mobilities were determined. The separations were characterized as to numbers of theoretical plates, selectivities, and resolution at different pH and applied voltage values. Numbers of theoretical plates ranged from about 400 to 3000. Selectivities ranged from about 0.1 to 0.4, and resolution values ranged from 0.8 to 2.3. Electrophoretic mobilities varied especially at the higher applied voltages.