IMMUNE-RECOGNITION OF SCHISTOSOMA-MANSONI PRIMARY SPOROCYSTS MAY REQUIRE SPECIFIC RECEPTORS ON BIOMPHALARIA-GLABRATA HEMOCYTES
IMMUNE-RECOGNITION OF SCHISTOSOMA-MANSONI PRIMARY SPOROCYSTS MAY REQUIRE SPECIFIC RECEPTORS ON BIOMPHALARIA-GLABRATA HEMOCYTES
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DOI：
10.1111/j.1365-3024.1984.tb00822.x
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发表时间：
1984-01-01
影响因子：
2.2
通讯作者：
STEPHENS, JA
中科院分区：
文献类型：
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作者：
BAYNE, CJ;LOKER, ES;STEPHENS, JA
细胞相互作用，当B.光滑血细胞包裹S.曼氏孢子囊。兔抗体（IgG），当结合到孢子囊表面的抗原，防止正常的细胞粘附（CA）的血细胞从敏感和耐药主机蜗牛。由于即使固定的孢子囊也会干扰Ca，因此该效应不是由于IgG刺激的寄生虫表面调节。用2种特异性重叠的抗血清和定量免疫荧光抗体技术（QIFAT），测定了将等量IgG置于孢子囊上所需的IgG浓度。在这些浓度下，CA受到的影响不同，这意味着干扰是由于结合的特定抗原，而不仅仅是由于IgG的存在。同样使用QIFAT，通过全FITC [异硫氰酸荧光素]标记的IgG确定需要多少来自抗孢子囊血清的F（ab“）2和IgG来阻断等量的抗原决定簇进入。表面抗原同样被封闭的孢子囊对血细胞同样不粘附，这支持了这样的观点，即隐蔽抗原的性质，而不是Fc部分或较大尺寸的完整IgG蛋白，是对CA的影响的原因。这些令人惊讶的结果暗示了蜗牛血细胞上特异性抗原结合位点的作用。
Cellular interactions, leading to cell-mediated cytotoxicity when B. glabrata hemocytes encapsulate S. mansoni sporocysts, were investigated. Rabbit antibodies (IgG), when bound to antigens on sporocyst surfaces, prevent the normal cytoadherence (CA) of hemocytes from both susceptible and resistant host snails. Since interference with Ca occurs with even fixed sporocysts, the effect is not due to IgG stimulated modulation of the parasite surface. Using 2 antisera with some overlapping specificities, and quantitative immunofluorescent antibody technique (QIFAT), concentrations of IgG needed to place equivalent amounts of IgG on the sporocysts were determined. At these concentrations, CA was affected differentially, implying that interference was due to the specific antigens bound, and not due simply to the presence of IgG. Also with QIFAT, how much F(ab'')2 and IgG from anti-sporocyst serum were needed to block an equivalent amount of antigenic determinants from access was determined by whole FITC [fluorescein isothiocyanate] labeled IgG. sporocysts whose surface antigens were equally blocked were equally unadherent for hemocytes, supporting the notion that the nature of obscured antigens, and neither the Fc portion nor the larger size of intact IgG protein, was responsible for the effect on CA. These surprising results imply a role for specific antigen binding sites on snail hemocytes.