A new approach to the biobleaching of flax pulp with laccase using natural mediators
A new approach to the biobleaching of flax pulp with laccase using natural mediators
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DOI：
10.1016/j.biortech.2010.01.057
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发表时间：
2010-06-01
影响因子：
11.4
通讯作者：
Vidal, Teresa
中科院分区：
文献类型：
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作者：
Fillat, Amanda;Colom, Josep F.;Vidal, Teresa
苯酚丁香醛 (SA)、乙酰丁香酮 (AS) 和对香豆酸 (PCA) 被用作天然漆酶介体，与来自朱砂密孔菌的漆酶结合使用来漂白亚麻纤维。在酶稳定性、纸浆和废水特性方面将它们的性能与 1-羟基苯并三唑 (HBT) 进行了比较。发现 HBT 和 PCA 在没有纸浆的情况下可使漆酶失活。然而，在未漂白亚麻浆存在的情况下，稳定性增加；例如，使用 PCA 时，漆酶保留了 77% 的初始活性，而在没有果肉的情况下漆酶则完全失活。这表明纸浆对酶变性具有保护作用。在随后用过氧化氢进行碱处理后，所有天然介体都会导致卡伯值降低； SA 的降低尤其显着（相对于对照样本约 2 个单位），并且与 HBT 获得的结果相当。所有天然介质均显着提高了亮度，但尤其是 AS 和 SA（两者均为 23%），其在这方面的表现与 HBT 非常相似。因此，天然介质可能是亚麻浆生物漂白合成介质的有效替代品。本文首次展示了天然介质在漆酶辅助亚麻浆脱木素中的使用及其对所得废水性质的影响。 (C) 2010 Elsevier Ltd. 保留所有权利。
The phenols syringaldehyde (SA), acetosyringone (AS) and p-coumaric acid (PCA) were used as natural laccase mediators in combination with a laccase from Pycnoporus cinnabarinus to bleach flax fibres. Their performance was compared with 1-hydroxybenzotriazole (HBT) in terms of enzyme stability, and pulp and effluent properties. HBT and PCA were found to inactivate laccase in the absence of pulp. However, in the presence of unbleached flax pulp stability was increased; for example with PCA, laccase retained 77% of its initial activity, in contrast with complete inactivation in the absence of pulp. This suggests a protective effect of the pulp against denaturalization of the enzyme. All natural mediators resulted in a reduced kappa number after the subsequent alkaline treatment with hydrogen peroxide; the reduction being especially marked with SA (about 2 units with respect to the control sample) and comparable to that obtained by HBT. Brightness was significantly increased by all natural mediators, but especially by AS and SA (23% with both), which performed very similarly to HBT in this respect. Natural mediators therefore might constitute an effective alternative to synthetic mediators for flax pulp biobleaching. This paper demonstrates for the first time the use of natural mediators in the laccase-assisted delignification of flax pulp and their effect on the properties of the resulting effluents. (C) 2010 Elsevier Ltd. All rights reserved.